nsP4

616 residues (nt 5751-7598), 69219.75 dalton

(The numbering used here for nsP4 residues start immediately after the opal codon. The protein is reputed to start at the Tyr, 7th residue from the opal codon).

Sequence (see also comparison with other alphaviruses)

             10         20         30         40         50
  0  LTGVGGYIFS TDTGPGHLQK KSVLQNQLTE PTLERNVLER IHAPVLDTSK
 50  EEQLKLRYQM MPTEANKSRY QSRKVENQKA ITTERLLSGL RLYNSATDQP
100  ECYKITYPKP LYSSSVPANY SDPQFAVAVC NNYLHENYPT VASYQITDEY
150  DAYLDMVDGT VACLDTATFC PAKLRSYPKK HEYRAPNIRS AVPSAMQNTL
200  QNVLIAATKR NCNVTQMREL PTLDSATFNV ECFRKYACND EYWEEFARKP
250  IRITTEFVTA YVARLKGPKA AALFAKTYNL VPLQEVPMDR FVMDMKRDVK
300  VTPGTKHTEE RPKVQVIQAA EPLATAYLCG IHRELVRRLT AVLLPNIHTL
350  FDMSAEDFDA IIAEHFKQGD PVLETDIASF DKSQDDAMAL TGLMILEDLG
400  VDQPLLDLIE CAFGEISSTH LPTGTRFKFG AMMKSGMFLT LFVNTVLNVV
450  IASRVLEERL KTSRCAAFIG DDNIIHGVVS DKEMAERCAT WLNMEVKIID
500  AVIGERPPYF CGGFILQDSV TSTACRVADP LKRLFKLGKP LPADDEQDED
550  RRRALLDETK AWFRVGITGT LAVAVTTRYE VDNITPVLLA LRTFAQSKRA
600  FQAIRGEIKH LYGGPK

Composition (mole %)

Ala 56 (9.1 %) Asx  0 (0.0 %) Cys 13 (2.1 %) Asp 37 (6.0 %) Glu 40 (6.5 %)
Phe 25 (4.1 %) Gly 28 (4.5 %) His 11 (1.8 %) Ile 30 (4.9 %) Lys 36 (5.8 %)
Leu 60 (9.7 %) Met 16 (2.6 %) Asn 23 (3.7 %) Pro 33 (5.4 %) Gln 23 (3.7 %)
Arg 38 (6.2 %) Ser 28 (4.5 %) Thr 49 (8.0 %) Val 44 (7.1 %) Trp  3 (0.5 %)
Tyr 23 (3.7 %) Glx  0 (0.0 %) xxx  0 (0.0 %)

Titration of nsP4 (number of residues protonated)

(-) (+) net pH Cys Asp Glu Tyr COOH charge His Lys Arg NH2 charge charge
1.0 13.00 36.95 39.98 23.00 1.00 -0.07 11.00 36.00 38.00 1.00 86.00 85.93
1.5 13.00 36.86 39.93 23.00 0.99 -0.22 11.00 36.00 38.00 1.00 86.00 85.78
2.0 13.00 36.55 39.78 23.00 0.97 -0.70 11.00 36.00 38.00 1.00 86.00 85.30
2.5 13.00 35.61 39.30 23.00 0.92 -2.17 11.00 36.00 38.00 1.00 86.00 83.83
3.0 13.00 32.95 37.87 23.00 0.78 -6.40 11.00 36.00 38.00 1.00 86.00 79.60
3.5 13.00 26.64 33.96 23.00 0.53 -16.87 10.99 36.00 38.00 1.00 85.99 69.12
4.0 13.00 16.59 25.60 23.00 0.27 -35.54 10.97 36.00 38.00 1.00 85.97 50.43
4.5 13.00 7.57 14.40 23.00 0.10 -55.93 10.89 36.00 38.00 1.00 85.89 29.96
5.0 12.99 2.78 6.04 23.00 0.04 -69.15 10.66 36.00 38.00 1.00 85.66 16.51
5.5 12.98 0.93 2.13 23.00 0.01 -74.95 10.00 36.00 38.00 1.00 85.00 10.05
6.0 12.94 0.30 0.70 23.00 0.00 -77.06 8.36 36.00 38.00 1.00 83.36 6.30
6.5 12.80 0.09 0.22 23.00 0.00 -77.89 5.50 36.00 38.00 0.99 80.49 2.60
7.0 12.38 0.03 0.07 23.00 0.00 -78.52 2.64 35.99 38.00 0.97 77.60 -0.92
7.5 11.22 0.01 0.02 22.99 0.00 -79.76 1.00 35.98 38.00 0.92 75.90 -3.86
8.0 8.66 0.00 0.01 22.97 0.00 -82.36 0.34 35.94 38.00 0.78 75.06 -7.30
8.5 5.03 0.00 0.00 22.92 0.00 -86.05 0.11 35.82 38.00 0.53 74.46 -11.59
9.0 2.16 0.00 0.00 22.74 0.00 -89.10 0.03 35.43 37.99 0.27 73.72 -15.38
9.5 0.77 0.00 0.00 22.21 0.00 -91.02 0.01 34.24 37.96 0.10 72.31 -18.71
10.0 0.25 0.00 0.00 20.68 0.00 -93.07 0.00 30.98 37.88 0.04 68.90 -24.17
10.5 0.08 0.00 0.00 16.98 0.00 -96.94 0.00 23.80 37.62 0.01 61.43 -35.51
11.0 0.03 0.00 0.00 10.84 0.00 -103.13 0.00 13.73 36.84 0.00 50.57 -52.56
11.5 0.01 0.00 0.00 5.06 0.00 -108.93 0.00 5.87 34.55 0.00 40.42 -68.51
12.0 0.00 0.00 0.00 1.88 0.00 -112.12 0.00 2.09 28.87 0.00 30.96 -81.16
12.5 0.00 0.00 0.00 0.63 0.00 -113.37 0.00 0.69 19.00 0.00 19.69 -93.68
13.0 0.00 0.00 0.00 0.20 0.00 -113.80 0.00 0.22 9.13 0.00 9.35 -104.45

Isoelectric point

                                           (-)                                (+)      net 
 pI    Cys    Asp    Glu    Tyr   COOH   charge   His    Lys    Arg    NH2  charge   charge
6.86  12.54   0.04   0.10  23.00  0.00   -78.32   3.34  36.00  38.00  0.98  78.32     0.00

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