nsP2

807 residues (nt 1680-4100), 89737.85 daltons

Sequence (see also comparison with other alphaviruses)

             10         20         30         40         50
  0  ALVETPRGHV RIIPQANDRM IGQYIVVSPN SVLKNAKLAP AHPLADQVKI
 50  ITHSGRSGRY AVEPYDAKVL MPAGGAVPWP EFLALSESAT LVYNEREFVN
100  RKLYHIAMHG PAKNTEEEQY KVTKAELAET EYVFDVDKKR CVKKEEASGL
150  VLSGELTNPP YHELALEGLK TRPAVPYKVE TIGVIGTPGS GKSAIIKSTV
200  TARDLVTSGK KENCREIEAD VLRLRGMQIT SKTVDSVMLN GCHKAVEVLY
250  VDEAFACHAG ALLALIAIVR PRKKVVLCGD PMQCGFFNMM QLKVHFNHPE
300  KDICTKTFYK YISRRCTQPV TAIVSTLHYD GKMKTTNPCK KNIEIDITGA
350  TKPKPGDIIL TCFRGWVKQL QIDYPGHEVM TAAASQGLTR KGVYAVRQKV
400  NENPLYAITS EHVNVLLTRT EDRLVWKTLQ GDPWIKQLTN IPKGNFQATI
450  EDWEAEHKGI IAAINSPTPR ANPFSCKTNV CWAKALEPIL ATAGIVLTGC
500  QWSELFPQFA DDKPHSAIYA LDVICIKFFG MDLTSGLFSK QSIPLTYHPA
550  DSARPVAHWD NSPGTRKYGY DHAIAAELSR RFPVFQLAGK GTQLDLQTGR
600  TRVISAQHNL VPVNRNLPHA LVPEYKEKQP GPVKKFLNQF KHHSVLVVSE
650  EKIEAPRKRI EWIAPIGIAG ADKNYNLAFG FPPQARYDLV FINIGTKYRN
700  HHFQQCEDHA ATLKTLSRSA LNCLNPGGTL VVKSYGYADR NSEDVVTALA
750  RKFVRVSAAR PDCVSSNTEM YLIFRQLDNS RTRQFTPHHL NCVISSVYEG
800  TRDGVGA

Composition (mole %)

Ala 72 (8.9 %) Asx  0 (0.0 %) Cys 18 (2.2 %) Asp 34 (4.2 %) Glu 45 (5.6 %)
Phe 27 (3.3 %) Gly 50 (6.2 %) His 27 (3.3 %) Ile 48 (5.9 %) Lys 58 (7.2 %)
Leu 64 (7.9 %) Met 12 (1.5 %) Asn 36 (4.5 %) Pro 49 (6.1 %) Gln 29 (3.6 %)
Arg 43 (5.3 %) Ser 41 (5.1 %) Thr 52 (6.4 %) Val 65 (8.1 %) Trp  9 (1.1 %)
Tyr 28 (3.5 %) Glx  0 (0.0 %) xxx  0 (0.0 %)

Titration of nsP2 (number of residues protonated)

(-) (+) net pH Cys Asp Glu Tyr COOH charge His Lys Arg NH2 charge charge
1.0 18.00 33.96 44.97 28.00 1.00 -0.07 27.00 58.00 43.00 1.00 129.00 128.93
1.5 18.00 33.87 44.92 28.00 0.99 -0.22 27.00 58.00 43.00 1.00 129.00 128.78
2.0 18.00 33.59 44.75 28.00 0.97 -0.69 27.00 58.00 43.00 1.00 129.00 128.31
2.5 18.00 32.73 44.21 28.00 0.92 -2.14 27.00 58.00 43.00 1.00 129.00 126.86
3.0 18.00 30.28 42.60 28.00 0.78 -6.34 26.99 58.00 43.00 1.00 128.99 122.65
3.5 18.00 24.48 38.21 28.00 0.53 -16.78 26.97 58.00 43.00 1.00 128.97 112.19
4.0 18.00 15.25 28.80 28.00 0.27 -35.68 26.91 58.00 43.00 1.00 128.91 93.23
4.5 18.00 6.95 16.20 28.00 0.10 -56.75 26.73 58.00 43.00 1.00 128.73 71.98
5.0 17.99 2.56 6.79 28.00 0.04 -70.62 26.17 58.00 43.00 1.00 128.17 57.55
5.5 17.97 0.85 2.40 28.00 0.01 -76.77 24.55 58.00 43.00 1.00 126.55 49.78
6.0 17.91 0.27 0.79 28.00 0.00 -79.03 20.51 58.00 43.00 1.00 122.51 43.48
6.5 17.72 0.09 0.25 28.00 0.00 -79.94 13.50 58.00 43.00 0.99 115.49 35.55
7.0 17.14 0.03 0.08 28.00 0.00 -80.75 6.49 57.99 43.00 0.97 108.45 27.70
7.5 15.54 0.01 0.03 27.99 0.00 -82.43 2.45 57.97 43.00 0.92 104.34 21.91
8.0 11.99 0.00 0.01 27.97 0.00 -86.03 0.83 57.91 43.00 0.78 102.52 16.49
8.5 6.96 0.00 0.00 27.90 0.00 -91.14 0.27 57.70 43.00 0.53 101.50 10.36
9.0 2.99 0.00 0.00 27.69 0.00 -95.32 0.09 57.07 42.99 0.27 100.42 5.10
9.5 1.07 0.00 0.00 27.04 0.00 -97.89 0.03 55.17 42.96 0.10 98.26 0.37
10.0 0.35 0.00 0.00 25.18 0.00 -100.47 0.01 49.91 42.86 0.04 92.82 -7.65
10.5 0.11 0.00 0.00 20.67 0.00 -105.22 0.00 38.34 42.57 0.01 80.92 -24.30
11.0 0.04 0.00 0.00 13.20 0.00 -112.76 0.00 22.12 41.68 0.00 63.80 -48.96
11.5 0.01 0.00 0.00 6.16 0.00 -119.83 0.00 9.46 39.09 0.00 48.55 -71.28
12.0 0.00 0.00 0.00 2.29 0.00 -123.71 0.00 3.37 32.67 0.00 36.04 -87.67
12.5 0.00 0.00 0.00 0.77 0.00 -125.23 0.00 1.11 21.50 0.00 22.61 -102.62
13.0 0.00 0.00 0.00 0.25 0.00 -125.75 0.00 0.36 10.33 0.00 10.69 -115.06

Isoelectric point

                                          (-)                                 (+)      net 
  pI    Cys    Asp    Glu    Tyr  COOH   charge    His   Lys    Arg    NH2  charge   charge
 9.53  0.99   0.00   0.00  26.97  0.00   -98.04   0.03  54.96  42.95  0.10   98.04     0.00

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Send suggestions and comments to: huang@borcim.wustl.edu WWW Department of Molecular Microbiology, Box 8230 Washington University School of Medicine St. Louis, MO 63110-1093 USA		Tel 314-362-2755 FAX 314-362-1232