nsP1

540 residues (nt 60-1679), 60363.03 daltons

Sequence (see also comparison with other alphaviruses)

             10         20         30         40         50
  0  MEKPVVNVDV DPQSPFVVQL QKSFPQFEVV AQQVTPNDHA NARAFSHLAS
 50  KLIELEVPTT ATILDIGSAP ARRMFSEHQY HCVCPMRSPE DPDRMMKYAS
100  KLAEKACKIT NKNLHEKIKD LRTVLDTPDA ETPSLCFHND VTCNMRAEYS
150  VMQDVYINAP GTIYHQAMKG VRTLYWIGFD TTQFMFSAMA GSYPAYNTNW
200  ADEKVLEARN IGLCSTKLSE GRTGKLSIMR KKELKPGSRV YFSVGSTLYP
250  EHRASLQSWH LPSVFHLNGK QSYTCRCDTV VSCEGYVVKK ITISPGITGE
300  TVGYAVTHNS EGFLLCKVTD TVKGERVSFP VCTYIPATIC DQMTGIMATD
350  ISPDDAQKLL VGLNQRIVIN GRTNRNTNTM QNYLLPIIAQ GFSKWAKERK
400  DDLDNEKMLG TRERKLTYGC LWAFRTKKVH SFYRPPGTQT CVKVPASFSA
450  FPMSSVWTTS LPMSLRQKLK LALQPKKEEK LLQVSEELVM EAKAAFEDAQ
500  EEARAEKLRE ALPPLVADKG IEAAAEVVCE VEGLQADIGA

Composition (mole %)

Ala 44 (8.1 %)   Asx  0 (0.0 %)   Cys 15 (2.8 %)   Asp 25 (4.6 %)   Glu 36 (6.7 %)
Phe 19 (3.5 %)   Gly 27 (5.0 %)   His 12 (2.2 %)   Ile 23 (4.3 %)   Lys 39 (7.2 %)
Leu 43 (8.0 %)   Met 18 (3.3 %)   Asn 20 (3.7 %)   Pro 30 (5.6 %)   Gln 23 (4.3 %)
Arg 26 (4.8 %)   Ser 36 (6.7 %)   Thr 39 (7.2 %)   Val 42 (7.8 %)   Trp  6 (1.1 %)
Tyr 17 (3.1 %)   Glx  0 (0.0 %)   xxx  0 (0.0 %)

Titration of nsP1 (number of residues protonated)

(-) (+) net pH Cys Asp Glu Tyr COOH charge His Lys Arg NH2 charge charge
1.0 15.00 24.97 35.98 17.00 1.00 -0.05 12.00 39.00 26.00 1.00 78.00 77.95
1.5 15.00 24.90 35.94 17.00 0.99 -0.17 12.00 39.00 26.00 1.00 78.00 77.83
2.0 15.00 24.70 35.80 17.00 0.97 -0.53 12.00 39.00 26.00 1.00 78.00 77.47
2.5 15.00 24.06 35.37 17.00 0.92 -1.65 12.00 39.00 26.00 1.00 78.00 76.35
3.0 15.00 22.26 34.08 17.00 0.78 -4.88 12.00 39.00 26.00 1.00 78.00 73.12
3.5 15.00 18.00 30.57 17.00 0.53 -12.90 11.99 39.00 26.00 1.00 77.99 65.09
4.0 15.00 11.21 23.04 17.00 0.27 -27.48 11.96 39.00 26.00 1.00 77.96 50.48
4.5 15.00 5.11 12.96 17.00 0.10 -43.83 11.88 39.00 26.00 1.00 77.88 34.05
5.0 14.99 1.88 5.44 17.00 0.04 -54.65 11.63 39.00 26.00 1.00 77.63 22.98
5.5 14.98 0.63 1.92 17.00 0.01 -59.46 10.91 39.00 26.00 1.00 76.91 17.45
6.0 14.93 0.20 0.63 17.00 0.00 -61.24 9.12 39.00 26.00 1.00 75.12 13.88
6.5 14.77 0.06 0.20 17.00 0.00 -61.97 6.00 39.00 26.00 0.99 71.99 10.02
7.0 14.28 0.02 0.06 17.00 0.00 -62.64 2.88 38.99 26.00 0.97 68.84 6.20
7.5 12.95 0.01 0.02 16.99 0.00 -64.03 1.09 38.98 26.00 0.92 66.99 2.96
8.0 9.99 0.00 0.01 16.98 0.00 -67.02 0.37 38.94 26.00 0.78 66.09 -0.93
8.5 5.80 0.00 0.00 16.94 0.00 -71.26 0.12 38.80 26.00 0.53 65.45 -5.81
9.0 2.50 0.00 0.00 16.81 0.00 -74.69 0.04 38.38 25.99 0.27 64.68 -10.01
9.5 0.89 0.00 0.00 16.42 0.00 -76.69 0.01 37.10 25.97 0.10 63.18 -13.51
10.0 0.29 0.00 0.00 15.29 0.00 -78.42 0.00 33.56 25.92 0.04 59.52 -18.90
10.5 0.09 0.00 0.00 12.55 0.00 -81.36 0.00 25.78 25.74 0.01 51.53 -29.83
11.0 0.03 0.00 0.00 8.01 0.00 -85.96 0.00 14.87 25.20 0.00 40.07 -45.89
11.5 0.01 0.00 0.00 3.74 0.00 -90.25 0.00 6.36 23.64 0.00 30.00 -60.25
12.0 0.00 0.00 0.00 1.39 0.00 -92.61 0.00 2.26 19.75 0.00 22.01 -70.60
12.5 0.00 0.00 0.00 0.47 0.00 -93.53 0.00 0.75 13.00 0.00 13.75 -79.78
13.0 0.00 0.00 0.00 0.15 0.00 -93.85 0.00 0.24 6.25 0.00 6.49 -87.36

Isoelectric point

                                          (-)                                (+)     net 
 pI    Cys    Asp    Glu    Tyr   COOH  charge   His    Lys    Arg    NH2  charge  charge
7.89  10.77   0.00   0.01  16.99  0.00  -66.23   0.46  38.95  26.00  0.82  66.23    0.00

Return to Overview of Sindbis virus
Send suggestions and comments to: huang@borcim.wustl.edu WWW Department of Molecular Microbiology, Box 8230 Washington University School of Medicine St. Louis, MO 63110-1093 USA		Tel 314-362-2755 FAX 314-362-1232