E3 protein

E3

64 residues, 6994.68 dalton

Sequence

             10         20         30         40         50
  0  SAAPLVTAMC LLGNVSFPCD RPPTCYTREP SRALDILEEN VNHEAYDTLL
 50  NAILRCGSSG RSKR

Composition (mole %)

Ala 6 ( 9.4 %) Asx 0 (0.0 %) Cys 4 (6.2 %) Asp 3 (4.7 %) Glu 4 (6.2 %)
Phe 1 ( 1.6 %) Gly 3 (4.7 %) His 1 (1.6 %) Ile 2 (3.1 %) Lys 1 (1.6 %)
Leu 8 (12.5 %) Met 1 (1.6 %) Asn 4 (6.2 %) Pro 5 (7.8 %) Gln 0 (0 %)
Arg 6 ( 9.4 %) Ser 6 (9.4 %) Thr 4 (6.2 %) Val 3 (4.7 %) Trp 0 (0 %)
Tyr 2 ( 3.1 %) Glx 0 (0.0 %) xxx 0 (0.0 %)

Titration of E3 (number of residues protonated)

(-) (+) net pH Cys Asp Glu Tyr COOH charge His Lys Arg NH2 charge charge
1.0 4.00 3.00 4.00 2.00 1.00 0.00 1.00 1.00 6.00 1.00 9.00 9.00
1.5 4.00 2.99 3.99 2.00 0.99 -0.03 1.00 1.00 6.00 1.00 9.00 8.97
2.0 4.00 2.96 3.98 2.00 0.97 -0.09 1.00 1.00 6.00 1.00 9.00 8.91
2.5 4.00 2.89 3.93 2.00 0.92 -0.26 1.00 1.00 6.00 1.00 9.00 8.74
3.0 4.00 2.67 3.79 2.00 0.78 -0.76 1.00 1.00 6.00 1.00 9.00 8.24
3.5 4.00 2.16 3.40 2.00 0.53 -1.91 1.00 1.00 6.00 1.00 9.00 7.09
4.0 4.00 1.35 2.56 2.00 0.27 -3.82 1.00 1.00 6.00 1.00 9.00 5.18
4.5 4.00 0.61 1.44 2.00 0.10 -5.85 0.99 1.00 6.00 1.00 8.99 3.14
5.0 4.00 0.23 0.60 2.00 0.04 -7.13 0.97 1.00 6.00 1.00 8.97 1.84
5.5 3.99 0.08 0.21 2.00 0.01 -7.71 0.91 1.00 6.00 1.00 8.91 1.20
6.0 3.98 0.02 0.07 2.00 0.00 -7.93 0.76 1.00 6.00 1.00 8.76 0.83
6.5 3.94 0.01 0.02 2.00 0.00 -8.03 0.50 1.00 6.00 0.99 8.49 0.46
7.0 3.81 0.00 0.01 2.00 0.00 -8.18 0.24 1.00 6.00 0.97 8.21 0.03
7.5 3.45 0.00 0.00 2.00 0.00 -8.55 0.09 1.00 6.00 0.92 8.01 -0.54
8.0 2.66 0.00 0.00 2.00 0.00 -9.34 0.03 1.00 6.00 0.78 7.81 -1.53
8.5 1.55 0.00 0.00 1.99 0.00 -10.46 0.01 0.99 6.00 0.53 7.53 -2.93
9.0 0.67 0.00 0.00 1.98 0.00 -11.35 0.00 0.98 6.00 0.27 7.25 -4.10
9.5 0.24 0.00 0.00 1.93 0.00 -11.83 0.00 0.95 5.99 0.10 7.04 -4.79
10.0 0.08 0.00 0.00 1.80 0.00 -12.12 0.00 0.86 5.98 0.04 6.88 -5.24
10.5 0.03 0.00 0.00 1.48 0.00 -12.49 0.00 0.66 5.94 0.01 6.61 -5.88
11.0 0.01 0.00 0.00 0.94 0.00 -13.05 0.00 0.38 5.82 0.00 6.20 -6.85
11.5 0.00 0.00 0.00 0.44 0.00 -13.56 0.00 0.16 5.45 0.00 5.61 -7.95
12.0 0.00 0.00 0.00 0.16 0.00 -13.84 0.00 0.06 4.56 0.00 4.62 -9.22
12.5 0.00 0.00 0.00 0.05 0.00 -13.95 0.00 0.02 3.00 0.00 3.02 -10.93
13.0 0.00 0.00 0.00 0.02 0.00 -13.98 0.00 0.01 1.44 0.00 1.45 -12.53

Isoelectric point

                                       (-)                           (+)     net 
 pI    Cys   Asp   Glu   Tyr  COOH   charge  His   Lys   Arg   NH2 charge  charge
7.03  3.79  0.00  0.01  2.00  0.00   -8.20  0.23  1.00  6.00  0.97  8.20    0.00

Return to Overview of Sindbis virus
Send suggestions and comments to: huang@borcim.wustl.edu WWW Department of Molecular Microbiology, Box 8230 Washington University School of Medicine St. Louis, MO 63110-1093 USA		Tel 314-362-2755 FAX 314-362-1232