E2

423 residues, 46914.81 dalton

Sequence

             10         20         30         40         50
  0  SVIDDFTLTS PYLGTCSYCH HTVPCFSPVK IEQVWDEADD NTIRIQTSAQ
 50  FGYDQSGAAS ANKYRYMSLK QDHTVKEGTM DDIKISTSGP CRRLSYKGYF
100  LLAKCPPGDS VTVSIVSSNS ATSCTLARKI KPKFVGREKY DLPPVHGKKI
150  PCTVYDRLKE TTAGYITMHR PRPHAYTSYL EESSGKVYAK PPSGKNITYE
200  CKCGDYKTGT VSTRTEITGC TAIKQCVAYK SDQTKWVFNS PDLIRHDDHT
250  AQGKLHLPFK LIPSTCMVPV AHAPNVIHGF KHISLQLDTD HLTLLTTRRL
300  GANPEPTTEW IVGKTVRNFT VDRDGLEYIW GNHEPVRVYA QESAPGDPHG
350  WPHEIVQHYY HRHPVYTILA VASATVAMMI GVTVAVLCAC KARRECLTPY
400  ALAPNAVIPT SLALLCCVRS ANA

Composition (mole %)

Ala 32 (7.6 %) Asx  0 (0.0 %) Cys 17 (4.0 %) Asp 22 (5.2 %) Glu 16 (3.8 %)
Phe  9 (2.1 %) Gly 24 (5.7 %) His 19 (4.5 %) Ile 23 (5.4 %) Lys 27 (6.4 %)
Leu 28 (6.6 %) Met  6 (1.4 %) Asn 11 (2.6 %) Pro 29 (6.9 %) Gln 11 (2.6 %)
Arg 20 (4.7 %) Ser 30 (7.1 %) Thr 40 (9.5 %) Val 32 (7.6 %) Trp  5 (1.2 %)
Tyr 22 (5.2 %) Glx  0 (0.0 %) xxx  0 (0.0 %)

Titration of E2 (number of residues protonated)

(-) (+) net pH Cys Asp Glu Tyr COOH charge His Lys Arg NH2 charge charge
1.0 17.00 21.97 15.99 22.00 1.00 -0.04 19.00 27.00 20.00 1.00 67.00 66.96
1.5 17.00 21.91 15.97 22.00 0.99 -0.13 19.00 27.00 20.00 1.00 67.00 66.87
2.0 17.00 21.73 15.91 22.00 0.97 -0.39 19.00 27.00 20.00 1.00 67.00 66.61
2.5 17.00 21.18 15.72 22.00 0.92 -1.18 19.00 27.00 20.00 1.00 67.00 65.82
3.0 17.00 19.59 15.15 22.00 0.78 -3.48 18.99 27.00 20.00 1.00 66.99 63.51
3.5 17.00 15.84 13.58 22.00 0.53 -9.05 18.98 27.00 20.00 1.00 66.98 57.93
4.0 17.00 9.87 10.24 22.00 0.27 -18.62 18.94 27.00 20.00 1.00 66.94 48.32
4.5 17.00 4.50 5.76 22.00 0.10 -28.64 18.81 27.00 20.00 1.00 66.81 38.17
5.0 16.99 1.65 2.42 22.00 0.04 -34.90 18.42 27.00 20.00 1.00 66.42 31.52
5.5 16.97 0.55 0.85 22.00 0.01 -37.62 17.27 27.00 20.00 1.00 65.27 27.65
6.0 16.92 0.18 0.28 22.00 0.00 -38.62 14.44 27.00 20.00 1.00 62.44 23.82
6.5 16.73 0.06 0.09 22.00 0.00 -39.12 9.50 27.00 20.00 0.99 57.49 18.37
7.0 16.19 0.02 0.03 22.00 0.00 -39.76 4.57 27.00 20.00 0.97 52.54 12.78
7.5 14.67 0.01 0.01 21.99 0.00 -41.32 1.73 26.99 20.00 0.92 49.64 8.32
8.0 11.32 0.00 0.00 21.98 0.00 -44.70 0.58 26.96 20.00 0.78 48.32 3.62
8.5 6.58 0.00 0.00 21.92 0.00 -49.50 0.19 26.86 20.00 0.53 47.58 -1.92
9.0 2.83 0.00 0.00 21.76 0.00 -53.41 0.06 26.57 19.99 0.27 46.89 -6.52
9.5 1.01 0.00 0.00 21.25 0.00 -55.74 0.02 25.68 19.98 0.10 45.78 -9.96
10.0 0.33 0.00 0.00 19.78 0.00 -57.89 0.01 23.23 19.94 0.04 43.22 -14.67
10.5 0.11 0.00 0.00 16.24 0.00 -61.65 0.00 17.85 19.80 0.01 37.66 -23.99
11.0 0.03 0.00 0.00 10.37 0.00 -67.60 0.00 10.30 19.39 0.00 29.69 -37.91
11.5 0.01 0.00 0.00 4.84 0.00 -73.15 0.00 4.41 18.18 0.00 22.59 -50.56
12.0 0.00 0.00 0.00 1.80 0.00 -76.20 0.00 1.57 15.20 0.00 16.77 -59.43
12.5 0.00 0.00 0.00 0.60 0.00 -77.40 0.00 0.52 10.00 0.00 10.52 -66.88
13.0 0.00 0.00 0.00 0.19 0.00 -77.81 0.00 0.17 4.81 0.00 4.98 -72.83

Isoelectric point:

                                          (-)                               (+)      net 
 pI     Cys    Asp    Glu   Tyr   COOH   charge   His   Lys    Arg    NH2  charge   charge
8.33   8.23   0.00   0.00  21.95  0.00  -47.82   0.28  26.91  20.00  0.63  47.82    0.00

Return to Overview of Sindbis virus
Send suggestions and comments to: huang@borcim.wustl.edu WWW Department of Molecular Microbiology, Box 8230 Washington University School of Medicine St. Louis, MO 63110-1093 USA		Tel 314-362-2755 FAX 314-362-1232