6K

55 residues, 6199.19 dalton

Sequence

             10         20         30         40         50
  0  ETFTETMSYL WSNSQPFFWV QLCIPLAAFI VLMRCCSCCL PFLVVAGAYL
 50  AKVDA

Composition (mole %)

Ala 6 (10.9 %) Asx 0 (0.0 %) Cys 5 (9.1 %) Asp 1 (1.8 %) Glu 2 (3.6 %)
Phe 5 ( 9.1 %) Gly 1 (1.8 %) His 0 (0.0 %) Ile 2 (3.6 %) Lys 1 (1.8 %)
Leu 7 (12.7 %) Met 2 (3.6 %) Asn 1 (1.8 %) Pro 3 (5.5 %) Gln 2 (3.6 %)
Arg 1 ( 1.8 %) Ser 4 (7.3 %) Thr 3 (5.5 %) Val 5 (9.1 %) Trp 2 (3.6 %)
Tyr 2 ( 3.6 %) Glx 0 (0.0 %) xxx 0 (0.0 %)

Titration of 6K (number of residues protonated)

(-) (+) net pH Cys Asp Glu Tyr COOH charge His Lys Arg NH2 charge charge
1.0 5.00 1.00 2.00 2.00 1.00 0.00 0.00 1.00 1.00 1.00 3.00 3.00
1.5 5.00 1.00 2.00 2.00 0.99 -0.01 0.00 1.00 1.00 1.00 3.00 2.99
2.0 5.00 0.99 1.99 2.00 0.97 -0.05 0.00 1.00 1.00 1.00 3.00 2.95
2.5 5.00 0.96 1.97 2.00 0.92 -0.15 0.00 1.00 1.00 1.00 3.00 2.85
3.0 5.00 0.89 1.89 2.00 0.78 -0.44 0.00 1.00 1.00 1.00 3.00 2.56
3.5 5.00 0.72 1.70 2.00 0.53 -1.05 0.00 1.00 1.00 1.00 3.00 1.95
4.0 5.00 0.45 1.28 2.00 0.27 -2.00 0.00 1.00 1.00 1.00 3.00 1.00
4.5 5.00 0.20 0.72 2.00 0.10 -2.98 0.00 1.00 1.00 1.00 3.00 0.02
5.0 5.00 0.08 0.30 2.00 0.04 -3.58 0.00 1.00 1.00 1.00 3.00 -0.58
5.5 4.99 0.03 0.11 2.00 0.01 -3.86 0.00 1.00 1.00 1.00 3.00 -0.86
6.0 4.98 0.01 0.03 2.00 0.00 -3.98 0.00 1.00 1.00 1.00 3.00 -0.98
6.5 4.92 0.00 0.01 2.00 0.00 -4.07 0.00 1.00 1.00 0.99 2.99 -1.08
7.0 4.76 0.00 0.00 2.00 0.00 -4.24 0.00 1.00 1.00 0.97 2.97 -1.27
7.5 4.32 0.00 0.00 2.00 0.00 -4.68 0.00 1.00 1.00 0.92 2.92 -1.76
8.0 3.33 0.00 0.00 2.00 0.00 -5.67 0.00 1.00 1.00 0.78 2.78 -2.89
8.5 1.93 0.00 0.00 1.99 0.00 -7.08 0.00 0.99 1.00 0.53 2.52 -4.56
9.0 0.83 0.00 0.00 1.98 0.00 -8.19 0.00 0.98 1.00 0.27 2.25 -5.94
9.5 0.30 0.00 0.00 1.93 0.00 -8.77 0.00 0.95 1.00 0.10 2.05 -6.72
10.0 0.10 0.00 0.00 1.80 0.00 -9.10 0.00 0.86 1.00 0.04 1.90 -7.20
10.5 0.03 0.00 0.00 1.48 0.00 -9.49 0.00 0.66 0.99 0.01 1.66 -7.83
11.0 0.01 0.00 0.00 0.94 0.00 -10.05 0.00 0.38 0.97 0.00 1.35 -8.70
11.5 0.00 0.00 0.00 0.44 0.00 -10.56 0.00 0.16 0.91 0.00 1.07 -9.49
12.0 0.00 0.00 0.00 0.16 0.00 -10.84 0.00 0.06 0.76 0.00 0.82 -10.02
12.5 0.00 0.00 0.00 0.05 0.00 -10.95 0.00 0.02 0.50 0.00 0.52 -10.43
13.0 0.00 0.00 0.00 0.02 0.00 -10.98 0.00 0.01 0.24 0.00 0.25 -10.73

Isoelectric point:

                                       (-)                           (+)     net 
 pI    Cys   Asp   Glu   Tyr  COOH   charge  His   Lys   Arg   NH2 charge  charge
4.51  5.00  0.20  0.70  2.00  0.10   -3.00  0.00  1.00  1.00  1.00  3.00    0.00

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