CURRICULUM VITAE

Samuel Leonard Stanley, Jr., M.D.

PERSONAL INFORMATION

Date of birth: January 11, 1954

Place of birth: Seattle, Washington

CITIZENSHIP: U.S.A.

ADDRESS:

OFFICE

Office of the Vice Chancellor for Research

Washington University in St. Louis

Campus Box 8027

660 S. Euclid Ave.

St. Louis, MO 63110

314-362-7010

PRESENT POSITION: Vice Chancellor for Research

EDUCATION:

1976 B.A., Biological Sciences, The College of the University of Chicago, IL

1980 M.D., Medicine, Harvard University Medical School, Cambridge, MA

1984-1987 Post-doctoral, Immunology, Washington University School of Medicine, St. Louis, MO

ACADEMIC POSITIONS/EMPLOYMENT:

1976 Teaching Assistant in Biology, The College of the University of Chicago, IL

1980-1981 Medical Intern, Massachusetts General Hospital, Boston, MA

1981-1983 Medical Resident, Massachusetts General Hospital, Boston, MA

1983-1984 Fellow in Infectious Diseases, Washington University School of Medicine, St. Louis, MO

1985-1988 Pfizer Fellow in Microbiology and Immunology, Washington University School of Medicine, St. Louis, MO

1987-1988 Instructor in Medicine, Washington University School of Medicine, St. Louis, MO

1988 Assistant Professor, Division of Infectious Diseases, Washington University School of Medicine, St. Louis, MO

1989 Assistant Professor, Department of Molecular Microbiology,Washington University School of Medicine, St. Louis, MO

1990-Present Chief Medical Consultant, BarnesCare Travelers Clinic

1993-1999 Associate Professor (with tenure), Department of Medicine

1994-2004 Associate Professor, Department of Molecular Microbiology

1999-Present Professor, Department of Medicine

2003-Present Director, Midwest Regional Center of Excellence for Biodefense and Emerging Infectious Diseases Research

2004-Present Professor, Department of Molecular Microbiology

2006-Present Vice Chancellor for Research

UNIVERSITY AND HOSPITAL APPOINTMENTS AND COMMITTEES:

1987- Present Attending physician, Internal Medicine and Infectious Diseases, Barnes-Jewish Hospital of St. Louis

Chief Medical Consultant, BarnesCare Travelers Clinic

1989 Chairman, Committee to formulate a health policy for Washington University Personnel who work with animals.

1992-2000 Member, Washington University MA/MD Committee

1992-2001 Program Committee, American Society of Tropical Medicine and Hygiene

1995-1997 At-large Representative Washington University Faculty Senate

Member, Senate Council of Washington University

Member, Advisory Committee on Academic Freedom and Tenure

1997-1999 Faculty Representative, Washington University Benefits Committee

1999-Present Selection Committee and Advisory Board Medical Student International Fellowships

2000-2002 Clinical Representative to the Executive Faculty, Washington University School of Medicine

2000-2004 Chairman, Institutional Biological and Chemical Safety Committee

2001-2002 Division of Biology and Biomedical Sciences Graduate Admissions Committee

MEDICAL LICENSURE AND BOARD CERTIFICATION:

Massachusetts License 1980-83

Missouri License 1983-present

American Board of Internal Medicine, Certification in Internal Medicine 1983

American Board of Internal Medicine, Certification in Infectious Diseases 1986

MILITARY SERVICE: None

HONORS:

1976 Honors in Biological Sciences, University of Chicago

1976 Phi Beta Kappa, University of Chicago

1979 Albert Schweitzer Fellow of Harvard Medical School

1985-1988 Pfizer Postdoctoral Fellow

1994-1999 Research Career Development Award, NIH

1999-2004 Burroughs-Wellcome Scholar in Molecular Parasitology

2000 Distinguished Service Teaching Award—Class of 2002

2002-2004 Permanent member, Tropical Medicine and Parasitology Study Section

2004-PresentPermanent member, Eukaryotic Pathogenesis Study Section

EDITORIAL RESPONSIBILITIES:

Editorial Board: Infection and Immunity 1998-

Ad hoc reviewer for:

New England Journal of Medicine Vaccine

Clinical Infectious Diseases Parasite Immunology

Journal of Infectious Diseases Experimental Parasitology

Molecular Microbiology Lancet

Gastroenterology Journal of Parasitology

Physiological Reviews Am.J.Tropical Medicine and Hygiene

Cellular Microbiology Laboratory Animal Science

PNAS Acta Tropica

Molecular and Biochemical Parasitology Nature

Ad hoc grant reviewer for:

Wellcome Trust

International Center for Diarrhoeal Disease Research USAID

American Federation for AIDS Research NIH—SEP

Temporary member: NIH-TMP study section 10-2000, 6-2002

2005—EpScor NSF Site Visit Team

PROFESSIONAL SOCIETIES AND ORGANIZATIONS:

Associate Member American College of Physicians, 1981

Member, Infectious Disease Society of America, 1989

Member, American Society of Tropical Medicine and Hygiene, 1988

Member, American Federation for Clinical Research, 1989

Member, American Society for Microbiology, 1992

Fellow, Infectious Disease Society of America, 1995

Member, American Society for Clinical Investigation, 1995

Secretary-Treasurer, Board of Directors, Infectious Diseases Society of St. Louis, 2004-present

Ambassador, Paul G. Rogers Society for Global Health Research, 2007-present

MAJOR INVITED LECTURES:

Visiting scientist and lecturer─Centro de Investigacion y de Estudios Avanzados del IPN, Mexico

City, Mexico 3/91

"Molecular approach to Entamoeba histolytica pathogenesis", St. Louis University 4/91.

Chair─Amebiasis Session, American Society Tropical Medicine Hygiene--"Isolation of an

Entamoeba histolytica cDNA clone encoding a protein with a zinc finger domain." Boston, 11/91.

Keynote Speaker─"Role of the amebic cysteine proteinase in amebic liver abscess formation". Meeting

of the Society of Biological Chemistry (Mexico), Zacetecas, Mexico, 11/94.

Co-chair and Speaker─Merck Symposium on Amebiasis--"New models for amebiasis". ASTMH

Meetings, Cincinatti. 11/94

Speaker─"Scid mouse model of amebiasis" and "Scid mice and gene knockout mice as models for

parasitic disease". India/U.S.A. Joint Vaccine Action Program, Lucknow, India. 12/94.

Speaker─"What can murine models tell us about the immunobiology of amebiasis?" Berne Immunology

Center, U. of Va., Charlottesville. 12/94

Speaker─"Progress in a vaccine for amebiasis.", Eur. Conf. Trop. Med., Hamburg, 11/95

Chair and Speaker─Symposium--New insights into the immunobiology of parasitic diseases from

knockout and scid mice. ASTMH Meeting, 12/96.

Speaker─Bernardo Sepulveda Molecular Biology Seminar, XIII Congress on Amebiasis, Mexico City,

Mexico. 1/97

Speaker─Keystone Symposium on Cellular and Molecular Cross Talk at Mucosal Surfaces,

Santa Fe, New Mexico 3/97.

Speaker─“EhADH2 enzyme: A novel target for anti-amebic drugs”. ICTDR Conference, Washington,

D.C., 4/97.

Speaker─“Oral and DNA vaccines to prevent amebiasis.” ICTDR Conference, Washington, D.C., 4/98.

Speaker─“How intestinal epithelial cells regulate the inflammatory response to enteric pathogens”.

U.T. Health Sciences Center, San Antonio, TX. 6/98

Visiting professor and speaker─“Amebiasis: Putting man into mouse to understand an ancient

enemy.” NYU Medical Center Grand Rounds. 1/99

Speaker─“Pathways for amebic induction of inflammation and programmed cell death”, Burroughs

Wellcome Symposium, ASTMH Meeting, Washington, D.C., 11/99.

Speaker─“Pathways for amoebic induction of inflammation and tissue damage.” International

Symposium on Amoebiasis. Hamburg, Germany, 7/2000.

Speaker─National Institutes of Health/National Institute for Allergic and Infectious Diseases “Amebic

dysentery and ICE.” 4/2001.

Speaker─St. Louis University “Amebic dysentery and ICE 9/2001

Speaker─U.T. El Paso “Amebic dysentery and ICE” 10/2001

Speaker─Southern Illinois University at Carbondale “Amebic dysentery and ICE”, 10/2001.

Speaker─Washington University School of Medicine, Department of Pediatrics Grand Rounds,

“Amebiasis—new insights into an ancient enemy” 10/2001

Chairman, and speaker: Session on Amebiasis: Ellison Foundation Conference on Tropical Diseases,

Bhubaneswar, India, Feb. 2002

Speaker─Woods Hole Tropical Medicine and Parasitology Course: “Amebiasis”, July 2002, July 2003.

Speaker—Plenary Session, X International Conference on Parasitology, Vancouver, B.C.

“Pathways for amebic induction of inflammation and programmed cell death. Aug. 2002.

Speaker—Special Symposium in Honor of Jean Hickman. ASTMH meeting Denver. “New insights into

amebiasis from SCID-HU-INT mice. Nov. 2002

Speaker—EMBO Conference on Amebiasis, Paris, France “Role of TNF in amebic induced

inflammation”. Mar. 2003

Speaker—Engineering Connections Series—“SARS and other emerging infectious diseases—the

dangers of a small world”. Sept. 2003

Speaker—Yonsei University—Challenges in the Post Genomic Era—“Simultaneous host/pathogen

genomics.” Nov. 2003.

Speaker—Korean Society of Parasitology—Pathways for amebic induction of inflammation and programmed cell death. Nov. 2003.

Speaker—Institute Pasteur—“New insights into dysentery from SCID-HU-INT mice”. Nov 2003.

Speaker—University of Illinois, Emerging Infectious Diseases Conference “Pathogenesis of amebiasis”.

Mar.2004.

Speaker—University of Pennsylvania, Parasitology Group: “Understanding amebiasisfrom the host and

pathogen perspective. Nov. 2004

Speaker—Washington University School of Medicine, Medical Grand Rounds: “Emerging Infectious

Diseases—Preparing for the Unexpected and the Inevitable”. Nov. 2004

Speaker—7^th Annual Conference on Hemophilia—San Juan Puerto Rico, Emerging Infections

—Preparing for the unexpected and the inevitable. Feb. 2005

Speaker—NIAID/NIDDK workship on Humanized Mouse Models of Disease. Washington, DC.

Speaker─ASM: "Chimeric SCID-Human Mice to Study Enteric Pathogens." Feb. 2006

Speaker─MMI/ID Seminar Series: "Molecular Dissection of Entamoeba Histolytica Pathogenesis."

Mar. 2006

Speaker─Washington University Reunion Medical Update: "Avian Influenza & Emerging Infectious

Diseases." May, 2006.

Speaker─Washington University Reunion College: "The Threat of Emerging Infectious Diseases, Avian

Influenza and Beyond." May, 2006

RESEARCH SUPPORT:

Principle Investigator, U54 AI057160-01 “Midwest Regional Center for Excellence in Biodefense and Emerging Infectious Diseases Research 9/1/03-3/1/08 Direct costs: $5,123,000/yr.

Principle Investigator, NIAID R01 AI-30084- "Molecular Dissection of Entamoeba histolytica pathogenesis." 8/01/00-5/31/2005 . Current year direct costs: $200,000

Principle Investigator, Pathways of inflammation and tissue damage in amebiasis. Burroughs Wellcome Scholar in Molecular Parasitology. 7/1/99 to 6/30/05. Total direct costs: $425,000.00

Principle Investigator, NIAID R01 AI-51621-01 “Structure-Function of Entamoeba alcohol dehydrogenase 2”. 5/01/02-3/31/06 Direct costs $200,000/yr.

CLINICAL TITLE AND RESPONSIBILITIES:

Attending physician, Red Medical Service, Barnes Hospital, 1989 to present.

Attending physician, Infectious Diseases Service, Barnes-Jewish Hospital, 1987 to present.

Chief Medical Consultant, BarnesCare Travelers Clinic, 1990 to present.

TEACHING TITLE AND RESPONSIBILITIES:

Lecturer, Washington University School of Medicine, 1st Year Course in Microbiology “Introduction to

Tropical Medicine”.

Lecturer, Washington University School of Medicine, 2nd Year Course in Pathophysiology of Infectious

Diseases "Bacteremia and Sepsis" and "Protozoa I, Protozoa III" “Cases in Tropical Medicine”

Lecturer, Infectious Diseases and the Diagnostic Laboratory Course, "Intestinal Protozoa".

Lecturer, Clinical Infectious Diseases Course, "Diarrheal Diseases", “Diseases of Travelers”, and

"Bacteremia and Sepsis”, “Tropical Diseases”.

Lecturer, Lucille P. Markey Special Emphasis Pathway in Human Pathobiology, “Vaccines for Malaria".

Lecturer, Microbial Pathogenesis Course, "MDR genes and pathogenesis".

Lecturer, Molecular Mechanisms of Disease Course, "Vaccines against parasitic diseases".

Instructor, Case Problems in Cell Biology and Biochemistry

Instructor, Tropical Medicine Course

Faculty advisor: International Health and Tropical Medicine Forum

Lecturer, Barnes Housestaff Conference, “Diseases of Travelers”.

Lecturer, Microbiology 1^st year Graduate Student Course: “Protozoan taxonomy and diversity”

PUBLICATIONS:

PEER-REVIEWED

1. Wong, YC; Stanley Jr, SL; Garber, BB. Separation and characterization of neuronal and glial cell populations from embryonic chick cerebra in culture. Anat. Anz. 1981; 150:351-373.

2. Stanley Jr, SL; Kehl, O. Ascending paralysis associated with diethylcarbamazine treatment of a M. loa loa infestation--A case report and review of the literature. Tropical Doctor 1982; 12:16-19.

3. Stanley Jr, SL; Lusk, R. Thoracic actinomycosis presenting as a brachial plexus syndrome. Thorax 1985: 40:74-75.

4. Powderly, WG; Stanley Jr, SL; Medoff, G. Pneumococcal endocarditis: Report of a series and review of the literature. Rev. Inf. Dis. 1986; 8:786-789.

5. Stanley Jr, SL; Bischoff, JK; Davie, JM. Antigen induced rheumatoid factors: Protein and carbohydrate antigens induce different rheumatoid factor responses. J. Immunol. 1987; 139:2936-2942.

6. Stanley Jr, SL; Li, E; Davie, JM. Antigen induced rheumatoid factors: Characterization of monoclonal rheumatoid factors produced after protein and carbohydrate immunization. Molecular Immunol. 1988; 25:285-291.

7. Li, E; Becker, A; Stanley Jr, SL. Use of Chinese hamster ovary cells with altered glycosylation patterns to define the carbohydrate specificity of Entamoeba histolytica adhesion. J.Exp.Med. 1988; 167:1725‑1730.

8. Li, E; Becker, A; Stanley Jr, SL. Chinese hamster ovary cells deficient in N‑acetylglycosaminyltransferase I activity are resistant to Entamoeba histolytica‑mediated cytotoxicity. Infect.Immun. 1989; 57:8‑12.

9. Stanley Jr, SL; Becker, A; Kunz‑Jenkins, C; Foster, L; Li, E. Cloning and expression of a membrane antigen of Entamoeba histolytica possessing multiple tandem repeats. Proc. Natl. Acad. Sci.USA 1990; 87:4976‑4980.

10. Burch, DJ; Li, E; Reed, S; Jackson, TFHG; Stanley Jr, SL. Isolation of a strain‑specific Entamoeba histolytica cDNA clone. J.Clin.Microbiol. 1991; 29:696‑701.

11. Stanley Jr, SL; Jackson, TFHG; Reed, SL; Calderon, J; Kunz‑Jenkins, C; Gathiram, V; Li, E. Serodiagnosis of invasive amebiasis using a recombinant Entamoeba histolytica protein. JAMA 1991; 266:1984‑1986.

12. Stanley Jr, SL; Foster, L; Phillips, N. Molecular analysis of carbohydrate antigen induced monoclonal IgM anti‑IgG antibodies (rheumatoid factors). Mol.Immunol. 1992; 29:453-61.

13. Stanley Jr, SL; Huizenga, H; Li, E. Isolation and partial characterization of a surface glycoconjugate of Entamoeba histolytica. Mol.Biochem.Parasitol. 1992; 50:127-138.

14. Stanley Jr, SL; Li, E. Isolation of an Entamoeba histolytica cDNA clone encoding a protein with a putative zinc finger domain. Mol.Biochem.Parasitol. 1992; 50:185-188.

15. Li, E; Kunz‑Jenkins, C; Stanley Jr, SL. Isolation and characterization of genomic clones encoding a serine‑rich Entamoeba histolytica protein. Mol.Biochem.Parasitol. 1992; 50:355-358.

16. Cieslak, PR; Stanley Jr, SL. Advances in amebiasis: implications for the clinician. Inf.Dis.Clin.Pract. 1992; 1(3):151-157.

17. Zhang, Y; Li, E; Jackson, TFHG; Zhang, T; Gathiram, V; Stanley Jr, SL. Use of a recombinant 170 kDa surface antigen of Entamoeba histolytica in serodiagnosis of amebiasis, and identification of immunodominant domains of the native molecule. J. Clin. Microbiol. 1992; 30:2788-2792.

18. Cieslak, PR; Virgin IV, HW; Stanley Jr, SL. A severe combined immunodeficient (SCID) mouse model for infection with Entamoeba histolytica. J. Exp. Med. 1992; 176:1605-1609.

19. Myung, K; Burch, DJ; Jackson, TFHG; Stanley Jr, SL. Serodiagnosis of invasive amebiasis using a recombinant Entamoeba histolytica-antigen based ELISA. Arch. Med. Res. 1992; 23:285-288.

20. Zhang, Y; Aley, S; Stanley Jr, SL; Gillin, FD. Cysteine-dependent zinc binding by membrane proteins of Giardia lamblia. Infect. Immun. 1993; 61:520-524.

21. Cieslak, PR; Zhang, T; Stanley Jr, SL. Expression of a recombinant Entamoeba histolytica antigen in a Salmonella typhimurium vaccine strain. Vaccine 1993; 11:773-776.

22. Zhang, Y; Li, E; Stanley Jr, SL. Entamoeba histolytica: The EHZc3 cDNA clone encodes a zinc-binding protein. Exp. Parasitol. 1993; 77:118-120.

23. Zhang, T; Cieslak, PR; Foster, L; Kunz-Jenkins, C; Stanley Jr, SL. Antibodies to the serine rich Entamoeba histolytica protein (SREHP) prevent amebic liver abscess in severe combined immunodeficient (SCID) mice. Parasite Immunol.1994; 16:225-230.

24. Zhang, T; Cieslak, PR; Stanley Jr, SL. Protection of gerbils from amebic liver abscess by immunization with a recombinan