Department of Molecular Microbiology, Washington University in St. Louis, School of Medicine

(photo)

Virginia L. Miller

Professor
Phone, office: 314 286-2891
FAX: 314 286-2896
email: virginia@borcim.wustl.edu

8260 McDonnell Pediatric Research Building
Box 8230, Dept. of Molecular Microbiology
Washington University School of Medicine
660 South Euclid Avenue
St. Louis, MO 63110 

Research Interests | CV | Seminars | Publications

Research Interests: Molecular and Genetic Analysis of Virulence of Yersinia and Klebsiella

My laboratory uses Yersinia, and Klebsiella as model systems to study bacterial pathogenesis. The long-term goals of our work are to understand the bacteria-host interaction at the molecular level, to learn how this interaction affects the pathogenesis of infections and to understand how these pathogens co-ordinate the expression of virulence determinants during an infection. To do this we use genetic, molecular and immunological approaches, in conjunction with the mouse model of infection.

We have applied several genetic approaches to identify new virulence genes of Y. enterocolitica and are currently characterizing these genes, their products, and their role in disease. We also have been studying the invasion gene inv, with a focus on understanding the mechanism of regulation of expression of inv and the co-ordination of its expression with other virulence genes. An inv regulatory gene, rovA, has been identified that regulates expression of inv in the laboratory and during an infection. RovA also regulates expression of other novel virulence determinants that influence the early inflammatory response to Yersinia infection. Recently we extended the analysis of RovA to Yersinia pestis, the causative agent of bubonic and pneumonic plague, and found that RovA is also required for full virulence of Y. pestis. Microarray analysis is being used to identify the RovA regulated genes in Y. pestis and Y. enterocolitica.

Type III secretion systems (T3SS) are a means by which Gram negative pathogens deliver effector proteins into host cells. One of the first, and best, characterized systems is encoded on the virulence plasmid of the yersiniae. However, a second T3SS was recently identified on the chromosome of Y. enterocolitica. We identified some of the key players in the regulation of expression of this system and have begun to identify the effectors secreted by the system. The long-term goals are to understand the role of this system and the individual effectors in the biology of Y. enterocolitica.

For Klebsiella we have developed a mouse model of infection using an intranasal inoculation method. A bank of 5,000 transposon mutants have been isolated and screened in this intranasal model of infection for mutations that alter the ability of Klebsiella to either colonize the lung or spread from the lung to the spleen. A subset of these mutants is currently being studied in more detail.


Students interested in Ph.D. Programs in Biology and Biomedical Sciences at Washington University should consult the following websites:

CV

Date and place of birth: April 28, 1958; Tripoli, Libya
Citizenship: U.S.A.

Present Position
Professor of the Departments of Molecular Microbiology and Pediatrics,
Washington University School of Medicine, 11/00 - present

Education
9/75 - 12/79 B.A.Biological Sciences, University of California, Santa Barbara
9/80 - 9/85 Ph.D.,Harvard University
Microbiology & Molecular Genetics 
Thesis Advisor: Dr. John J. Mekalanos
10/85 - 6/88 Postdoctoral Fellow at Stanford University 
Medical Microbiology
PI: Dr. Stanley Falkow

Academic Positions/Employment
6/88 - 7/94 Assistant Professor
Dept. Microbiology & Molecular Genetics and the Molecular Biology Institute, UCLA
 
7/94 - 12/96		 Associate Professor
Dept. of Microbiology & Molecular Genetics and the Molecular Biology Institute, UCLA
10/96 - 10/00 Associate Professor
Depts. of Pediatrics and Molecular Microbiology, Washington University School of Medicine

Honors and Awards
9/75-12/79 Chancellor's Scholarship, University of California at Santa Barbara
8/80-6/83 National Research Service Award, Harvard University
10/85-6/88 Damon Runyon-Walter Winchell Cancer Fund, Stanford University
7/89-6/93 Pew Scholar in Biomedical Sciences
7/94-6/99 Research Career Development Award, National Institutes of Health
7/01 - 6/03 ASM Foundation Lecturer
7/02-6/05 Nat Sternberg Prize Committee
elected 2003 American Academy of Microbiology Fellow

Editorial and Review Responsibilities
J. Bacteriology - Editorial Board Member (1992 -1997)
Infection & Immunity - Editorial Board Member (1994-2002)
International Journal of Medical Microbiology - Advisory Board (1999-present)
Current Opinion in Microbiology - Editorial Board Member (1998-present)
Molecular Microbiology - Senior Editor (1993 - 2006)
Faculty of 1000 (2001 - present)
Science - Board of Reviewing Editors (2005 - present)
Ad hoc reviewer for: J. Clinical Microbiology, Microbial Pathogenesis, Proc. Natl. Acad. Sci. USA, Science, Vaccine, J. Biological Chemistry, Trends in Microbiology, J. Exp. Medicine, NIH, USDA-SBIR, National Science Foundation, Veterans Administration, Wellcome Trust, Thrasher Research Fund, Swiss National Science Foundation, Idaho State Board of Education, W. M. Keck Foundation


Meetings Organized
Keystone Meeting "Molecular Events in Microbial Pathogenesis", Santa Fe, NM January 1994
Midwest Microbial Pathogenesis Meeting, St. Louis, MO September 1998
5th FASEB meeting on Microbial Pathogenesis, (Vice Chair), Snowmass, CO August 2002
6th FASEB meeting on Microbial Pathogenesis, (Chair), Snowmass, CO August 2004
4th ASM Biodefense Research Meeting, (Vice Chair), Washington, DC February 2006
5th ASM Biodefense Research Meeting (Co-Chair), Washington, DC February 2007

Professional Societies and Organizations
1986 - present Member, American Society for Microbiology
1986 - present Member, American Association for the Advancement of Science
7/05 - 6/08 American Academy of Microbiology, Distinguished Service Award Selection Committee (Chair)

Publications - Peer Reviewed

  1. Miller, V. L., and J. J. Mekalanos. 1984. Synthesis of cholera toxin is positively regulated at the transcriptional level by toxR. Proc. Natl. Acad. Sci. USA 81:3471-3475.
  2. Miller, V. L., and J. J. Mekalanos. 1985. Genetic analysis of the cholera toxin positive regulatory gene toxR. J. Bacteriol. 163:580-585.
  3. Miller, V. L., R. K. Taylor, and J. J. Mekalanos. 1986. The cholera toxin transcriptional activator ToxR is a transmembrane DNA binding protein. Cell 48:271-279.
  4. Taylor, R. K., V. L. Miller, D. Furlong, and J. J. Mekalanos. 1987. Use of phoA gene fusions to identify a pilus colonization factor coordinately regulated with cholera toxin. Proc. Natl. Acad. Sci. USA 84:2833-2837.
  5. Miller, V. L., and S. Falkow. 1988. Evidence for two genetic loci from Y. enterocolitica that can promote invasion of epithelial cells. Infect. Immun. 56:1242-1248.
  6. Miller, V. L., and J. J. Mekalanos. 1988. Use of a novel suicide vector in the construction of site directed mutations: Evidence that osmoregulation of virulence gene expression requires toxR. J. Bacteriol. 170:2575-2583.
  7. Miller, V. L., J. J. Farmer III, W. E. Hill, and S. Falkow. 1989. The ail locus is found uniquely in Y. enterocolitica serotypes commonly associated with disease. Infect. Immun. 57:121-131.
  8. Miller, V. L., V. J. DiRita, and J. J. Mekalanos. 1989. Identification of toxS, a regulatory gene whose product enhances toxR mediated activation of the cholera toxin promoter. J. Bacteriol. 171:1288-1293.
  9. Robins-Browne, R. M., M. D. Miliotis, S. Cianciosi. V. L. Miller, S. Falkow, and J. G. Morris, Jr. 1989. Evaluation of DNA colony hybridization and other techniques for detection of virulence in Yersinia spp. J. Clin. Microbiol. 27:644-650.
  10. Miller, V. L., J. B. Bliska, and S. Falkow. 1990. Nucleotide sequence of the Y. enterocolitica ail gene and identification of the protein product. J. Bacteriol. 172:1062-1069.
  11. Pepe, J. C., and V. L. Miller. 1990. The Yersinia enterocolitica inv gene product is an outer membrane protein that shares epitopes with Yersinia pseudotuberculosis invasin. J. Bacteriol. 172:3780-3789.
  12. Young, V. B., V. L. Miller, S. Falkow, and G. K. Schoolnik. 1990. Sequence, localization and function of the invasion protein of Yersinia enterocolitica. Mol. Microbiol. 4:1119-1128.
  13. Beer, K. B., and V. L. Miller. 1992. Amino acid substitutions in naturally occurring variants of Ail result in altered invasion activity. J. Bacteriol. 174:1360-1369.
  14. Krogstad, P., P. M. Mendelman, V. L. Miller, C. Clausen, S. Abbott, S. Weagant, C. Wilson, and D. B. Lewis. 1992. Cutaneous infection with Yersinia enterocolitica: Clinical and microbiological characteristics. J. Infect. Dis. 164:740-743.
  15. Stone, B. J., C. M. Garcia, J. L. Badger, T. Hassett, R. I. F. Smith, and V. L. Miller. 1992. Identification of novel loci affecting entry of Salmonella enteritidis into eukaryotic cells. J. Bacteriol. 174:3945-3952.
  16. Miller, V. L., K. B. Beer, W. P. Loomis, J. A. Olson, and S. I. Miller. 1992. An unusual pagC: :Tn phoA fusion leads to an invasion and virulence defective phenotype in Salmonella. Infect. Immun. 60:3763-3770.
  17. Farmer III, J. J., G. P. Carter, V. L. Miller, S. Falkow, and I. K. Wachsmuth. 1992. Pyrazinamidase, CR-MOX agar, salicin fermentation-esculin hydrolysis, and D-xylose fermentation for identifying pathogenic serotypes of Yersinia enterocolitica. J. Clin. Microbiol. 30:2589-2594.
  18. Valone, S. E., G. Chikami, and V. L. Miller. 1993. Stress induction of the virulence proteins (SPV A, B, and C) from native plasmid pSLD2 of Salmonella dublin. Infect. Immun. 61:705-713.
  19. Pepe, J. C., and V. L. Miller. 1993. Yersinia enterocolitica invasin: a primary role in the initiation of infection. Proc. Natl. Acad. Sci. USA 90:6473-6477.
  20. Kinder, S. A., J. L. Badger, G. O. Bryant, J. C. Pepe, and V. L. Miller. 1993. Cloning of the Yen I restriction endonuclease and methyltransferase from Yersinia enterocolitica serotype O8 and construction of a R-M+ mutant. Gene 136:271-275.
  21. Pepe, J. C., J. L. Badger, and V. L. Miller. 1994. Growth phase and low pH affect the thermal regulation of the Yersinia enterocolitica inv gene. Mol. Microbiol. 11:123-135.
  22. Mixter, P. F., V. Camerini, B. J. Stone, V. L. Miller, and M. Kronenberg. 1994. Mouse T lymphocytes that express a gd T-cell antigen receptor contribute to resistance to Salmonella infection in vivo. Infect. Immun. 62:4618-4621.
  23. Stone, B. J., and V. L. Miller. 1995. Salmonella enteritidis has a homologue of tolC and is required for virulence in BALB/c mice. Mol. Microbiol. 17:701-712.
  24. Wachtel, M. R., and V. L. Miller. 1995. In vivo and in vitro characterization of an ail mutant of Yersinia enterocolitica. Infect. Immun. 63:2541-2548.
  25. Badger, J. L., and V. L. Miller. 1995. Role of RpoS in survival of Yersinia enterocolitica to a variety of environmental stresses. J. Bacteriol. 177:5370-5373.
  26. Pepe, J. C., M. R. Wachtel, E. Wagar, and V. L. Miller. 1995. Pathogenesis of invasion mutants of Yersinia enterocolitica i n a BALB/c mouse model of infection. Infect. Immun. 63:4837-4848.
  27. Kapatral, V., J. A. Olson, J. C. Pepe, V. L. Miller, and S. A. Minnich. 1996. Temperature-dependent regulation of Yersinia enterocolitica class III flagellar genes. Mol. Microbiol. 19:1061-1071.
  28. Young, G. M., D. Amid, and V. L. Miller. 1996. A bifunctional urease enhances survival of pathogenic Yersinia enterocolitica and Morganella morganii at low pH. J. Bacteriol. 178:6487-6495.
  29. Han, Y. H., and V. L. Miller. 1997. A re-evaluation of the virulence phenotype of the inv yadA double mutants of Yersinia pseudotuberucolsis. Infect. Immun. 65:327-330.
  30. Young, Glenn M., and V. L. Miller. 1997. Identification of novel chromosomal loci affecting Yersinia enterocolitica pathogenesis. Mol. Microbiol. 25:319-328.
  31. Badger, J. L., and V. L. Miller. 1998. Expression of invasin and motility are coordinately regulated in Yersinia enterocolitica. J. Bacteriol. 180: 793-800.
  32. Hong, K. H., and V. L. Miller. 1998. Identification of a novel Salmonella invasion locus homologous to Shigella sp. ipgDE. J. Bacteriol. 180: 1793-1802.
  33. Schmiel, D. H., E. Wagar, L. Karamanou, D. Weeks, and V. L. Miller. 1998. Phospholipase A of Yersinia enterocolitica contributes to pathogenesis in a mouse model. Infect. Immun. 66:3941-3951.
  34. Darwin, A. J. and V. L. Miller. 1999. Identification of Yersinia enterocolitica genes affecting survival in an animal host using signature-tagged transposon mutagenesis. Mol. Microbiol. 32:51-62.
  35. Young, G. M., M. J. Smith, S. A. Minnich, and V. L. Miller. 1999. The Yersinia enterocolitica motility master regulatory operon, flhDC, is required for flagellin production, swimming motility and swarming motility. J. Bacteriol. 181(9):2823-2833.
  36. Young, G. M., D. H. Schmiel, and V. L. Miller. 1999. A new pathway for the secretion of virulence factors by bacteria: the flagellar export apparatus functions as a protein secretion system. Proc. Natl. Acad. Sci. USA. 96:6456-6461.
  37. Darwin, K. H. and V. L. Miller. 1999. InvF is required for the expression of genes encoding proteins secreted by the SPI1 type III secretion apparatus in Salmonella typhimurium. J. Bacteriol. 181:4949-4954.
  38. Revell, P. A. and V. L. Miller. 2000. A chromosomally encoded regulator is required for expression of the Y. enterocolitica inv gene and for virulence. Mol. Microbiol. 35:677-685.
  39. Darwin, K. H. and V. L. Miller. 2000. The putative invasion protein chaperone SicA acts together with InvF to activate the expression of Salmonella typhimurium virulence genes. Mol. Microbiol. 35:949-959.
  40. Schmiel, D. H., G. M. Young, and V. L. Miller. 2000. The Yersinia enterocolitica phospholipase gene yplA is part of the flagellar regulon. J. Bacteriol. 182:2314-2320.
  41. Young, G. M., J. L. Badger, and V. L. Miller. 2000. Motility is required to initiate host cell invasion by Yersinia enterocolitica. Infect. Immun. 68:4323-4326.
  42. Kugler, S, B. Young, V. L. Miller, and W. E. Goldman. 2000. Monitoring phase-specific gene expression in Histoplasma capsulatum with telomeric GFP fusion plasmids. Cell. Microbiol. 2:537-547.
  43. Badger, J. L., B Young, A. J. Darwin, and V. L. Miller. 2000. Yersinia enterocolitica ClpB affects levels of invasin and motility. J. Bacteriol. 182:5563-5571.
  44. Gort, A. S., and V. L. Miller. 2000. Identification and characterization of Yersinia enterocolitica genes induced during systemic infection. Infect. Immun. 68: 6633-6642.
  45. Darwin, A. J. and V. L. Miller. 2001. The psp locus of Yersinia enterocolitica is required for virulence and for growth in vitro when the Ysc type III secretion system is produced. Mol. Microbiol. 39:429-444.
  46. Darwin, K. H., L. S. Robinson, and V. L. Miller. 2001. SigE is a chaperone for the Salmonella typhimurium invasion protein Sig D. J. Bacteriol. 183:1452-1454.
  47. Darwin, K. H. and V. L. Miller. 2001. Type III secretion chaperone-dependent regulation: activation of virulence genes by SicA and InvF in Salmonella typhimurium. EMBO J. 20:1850-1862.
  48. Heusipp, G., G. M. Young, and V. L. Miller. 2001. HreP, an in vivo expressed protease of Yersinia enterocolitica, is a new member of the family of subtilisn/kexin-like proteases. J. Bacteriol. 183:3556-3563.
  49. Nelson, K. M., G. M. Young, and V. L. Miller. 2001. Identification of a locus involved in systemic dissemination of Yersinia enterocolitica. Infect. Immun.  69:6201-6208.
  50. Miller, V. L., K. B. Beer, G. Heusipp, B. M. Young, and M. R. Wachtel. 2001. Identification of regions of Ail required for the invasion and serum resistance phenotypes. Mol. Microbiol. 41:1053-1062.
  51. Dube, P. H., P. A. Revell, D. D. Chapli, R. G. Lorenz, and V. L. Miller. 2001. A role for IL-1a in inducing pathologic inflammation during bacterial infection. Proc. Natl. Acad. Sci. USA 98:10880-10885.
  52. Dube, P. H., S. Handley, P. A. Revell, and V. L. Miller. 2003. The rovA mutant of Y. enterocolitica displays differential degrees of virulence depending upon the route of infection. Infect. Immun. 71:3512-3520.
  53. Heusipp, G., M. A. Schmidt, and V. L. Miller. 2003. Identification of rpoE and nadB as host responsive elements of Yersinia enterocolitica. FEMS Micro. Lett. 226:291-298.
  54. Neuhaus, K., N. Anastasov, V. Kaberdin, K. P. Francis, V. L. Miller, and S. Scherer. 2003. The AGUAAA motif in cspA1/A2 mRNA is important for adaptation of Yersinia enterocolitica after cold shock. Mol. Microbiol. 50:1629-1645.
  55. Ellison, D. W., B. Young, K. Nelson, and V. L. Miller. 2003. YmoA negatively regulates expression of Invasin from Yersinia enterocolitica. J. Bacteriol. 185:7153-7159.
  56. Handley, S. A., P. H. Dube, P. A. Revell, and V. L. Miller. 2004. Characterization of oral Yersinia enterocolitica infection in three different strains of inbred mice. Infect. Immun. 72:1645-1656.
  57. Heusipp, G., K. M. Nelson, M. A. Schmidt, and V. L. Miller. 2004. Regulation of htrA expression in Yersinia enterocolitica. FEMS Micro. Lett. 231:227-235.
  58. Dube, P. H., S. A. Handley, J. Lewis, and V. L. Miller. 2004. Protective role of IL-6 during Y. enterocolitica infection is mediated through the modulation of inflammatory cytokines. Infect. Immun. 72:3561-3570.
  59. Walker, K. A., and V. L. Miller. 2004. Regulation of the Ysa type III secretion system of Yersinia enterocolitica by YsaE/SycB and YsrS/YsrR. J. Bacteriol. 186:4056-4066.
  60. Handley, S. A., R. D. Newberry, and V. L. Miller. 2005. Yersinia enterocolitica invasin dependent and independent mechanisms of systemic dissemination. Infect. Immun. 73:8453-8455
  61. Lawlor, M. S., J. Hsu, P. D. Rick, and V. L. Miller. 2005. Identification of Klebsiella pneumoniae virulence determinants using an intranasal infection model. Mol. Microbiol. 58:1054-1073.
  62. Lathem, W. W., S. D. Crosby, V. L. Miller, and W. E. Goldman. 2005. Progression of primary pneumonic plague: A mouse model of infection, pathology, and bacterial transcriptional activity. Proc. Natl. Acad. Sci. USA 102: 17786-17791.
  63. Mildiner-Earley, S., and V. L. Miller. 2006. Characterization of a novel porin involved in systemic Yersinia enterocolitica infection. Infect. Immun. 74:4361-4365.
  64. Handley, S. A., P. H. Dube, and V. L. Miller. 2006. Histamine signaling through the H2 receptor in the Peyer’s Patch is important for controlling Yersinia enterocolitica infection. Proc. Natl. Acad. Sci. USA 103:9268-9273. [Commentary in same issue by L. Hoffman and S. Miller, pg. 9377-9378]
  65. Ellison, D. W. and V. L. Miller. 2006. H-NS represses inv transcription in Yersinia enterocolitica through competition with RovA and interaction with YmoA. J. Bacteriol.188:5101-5112.
  66. Lawlor, M. S., S. A. Handley, and V. L. Miller. 2006. Comparison of the host response to wild type and cpsB mutant Klebsiella pneumoniae infections. Infect. Immun. 74:5402-5407.
  67. Cathelyn, J. S., S. D. Crosby, W. W. Lathem, W. E. Goldman, and V. L. Miller. 2006. RovA a global regulator of Y. pestis specifically required for bubonic plague. Proc. Natl. Acad. Sci. USA 103:13514-13519.
  68. Axler-DiPerte, G. L., V. L. Miller, and A. J. Darwin. 2006. YtxR, a conserved LysR-like regulator that induces the expression of genes encoding a putative ADP-ribosyltransferase toxin homologue in Yersinia enterocolitica. J. Bacteriol. 188:8033-8044.
  69. Lathem, W. W., P. A. Price, V. L. Miller, and W. E. Goldman. 2007. A plasminogen-activating protease specifically controls the development of primary pneumonic plague. Science 315:509-513. [Faculty of 1000 pick]
  70. Mildiner-Earley, S., K. A. Walker and V. L. Miller. 2007. Environmental stimuli identified affecting expression of the Ysa type three secretion locus. Adv. Exp. Med. Biol. (in press)
  71. Lawlor, M. S., C. O'Connor, and V. L. Miller. 2007. Yersiniabactin is a virulence factor for Klebsiella pneumoniae during pulmonary infection. Infect. Immun. 75:1463-1472.
  72. Handley, S. A., and V. L. Miller. 2007. General and specific host responses to bacterial infection in Peyer's patches: A role for stromelysin-1 (matrix metalloproteinase-3) during Salmonella enterica infection. Mol. Microbiol. 64:94-110.
  73. Barton, E. S., D. W. White, J. S. Cathelyn, K. A. Brett-McClellan, M. Engle, M. S. Diamond, V. L. Miller, and H. W. Virgin IV. 2007. Herpesvirus latency protects the host from bacterial infection: Latency as mutualistic symbiosis. Nature 447:326-329. [Faculty of 1000 pick]
  74. Lawrenz, M. and V. L. Miller. 2007. Comparitive analysis of the regulation of rovA from the pathogenic Yersiniae. J. Bacteriol. (in press)

Publications - Review articles, Book chapters, Symposium reports, Books edited

  1. Mekalanos, J. J., G. D. N. Pearson, D. J. Swartz, V. L. Miller, and I. Sporecke. 1983. Cholera toxin genes: Detailed genetic analysis and construction of vaccine strains. IN: Proceeding of the 19th Joint Conference of Cholera (National Institutes of Health, Bethesda, MD).
  2. Mekalanos, J. J., V. L. Miller, R. K. Taylor, I. Goldberg, and G. D. N. Pearson. 1984. Regulation of cholera toxin synthesis. IN: Proceeding of the 20th Joint Conference on Cholera (National Institutes of Health, Bethesda, MD).
  3. Mekalanos, J., I. Goldberg, V. Miller, G. Pearson, D. Swartz, R. Taylor, N. Harford, A. M. Gathoye, E. Simoen, B. Boon, and M. DeWilde. 1985. Genetic construction of cholera vaccine prototypes. IN: Lerner, R., Chanock, R., and Brown, F., eds. Vaccines 85, Cold Spring Harbor press. pp 101-105.
  4. Mekalanos, J. J., G. D. N. Pearson, D. J. Swartz, V. Miller, I. Sporecke, N. Harford, F. Groyne, and M. deWilde. 1986. Cholera toxin genes: detailed genetic analysis and construction of vaccine strains. IN: Advances in research on cholera and related diarrheas 3. eds. S. Kuwahara and N. F. Pierce. KTK Scientific Publishers, Tokyo.
  5. Mekalanos, J., V. Miller, R. Taylor, and I. Goldberg. 1986. Genetics of cholera toxin. IN: Protein-carbohydrate interactions in biological systems. Academic Press Ltd., London.
  6. Betley, M. J., V. L. Miller, and J. J. Mekalanos. 1986. Genetics of bacterial enterotoxins. Ann. Rev. Microbiol. 40:577-606.
  7. Peterson, K. M., R. K. Taylor, V. L. Miller, V. J. DiRita, and J. J. Mekalanos. 1988. Coordinate regulation of virulence determinants in Vibrio cholerae. IN: Molecular biology and infectious diseases. Elsevier, Paris. pp 165-172.
  8. Mekalanos, J. J., V. L. Miller, R. K. Taylor, I. Goldberg, and G. D. N. Pearson. 1988. Regulation of cholera toxin synthesis. IN: Advances in research on cholera and related diarrheas 4. eds. S. Kuwahara and N. F. Pierce. KTK Scientific Publishers, Tokyo. pp 247-258.
  9. Miller, V. L., B. B. Finlay, and S. Falkow. 1988. Factors essential for the penetration of mammalian cells by Yersinia. Curr. Top. Microbiol. Immunol. 138:15-39.
  10. Miller, V. L. 1992. Yersinia invasion genes and their products. ASM News 58:26-33.
  11. Miller, V. L. 1993. Bacterial-host cell interactions in Monterey. Trends in Microbiol. 1:79-81.
  12. Galan, J., V. L. Miller, and D. Portnoy. 1993. Discussion of in vitro and in vivo assays for studying bacterial entry into and survival within eukaryotic cells. Infectious Agents and Disease 2:288-290.
  13. Pepe, J. C., and V. L. Miller. 1993. The biological role of invasin during a Yersinia enterocolitica infection. Infectious Agents and Disease 2:236-241.
  14. Miller, V. L., and B. J. Stone. 1994. Molecular cloning of invasion genes from Yersinia and Salmonella. Meth. Enzymol. 236:546-551.
  15. Miller, V. L., and J. C. Pepe. 1994. The invasion genes of Yersinia: inv, ail and yadA. Bailliereís Clinical Infectious Diseases. 1:213-226.
  16. Molecular Genetics of Bacterial Pathogenesis. 1994. Edited by: V. L. Miller, J. B. Kaper, D. A. Portnoy, and R. R. Isberg. ASM Press, Washington, D. C. 529p.
  17. Miller, V. L. 1995. Tissue culture invasion - fact or artifact? Trends in Microbiol. 3:69-71.
  18. Bacterial Invasiveness. 1996. Edited by: V. L. Miller. Curr. Top. Microbiol. Immunol. vol. 209.
  19. Darwin, H. and V. L. Miller. 1999. Molecular basis of the interaction of Salmonella with the intestinal mucosa. Clin. Microbiol. Rev. 12:405-428.
  20. Miller, V. L. 1999. Signature-tagged mutagenesis and the hunt for virulence factors: Response. Trends in Microbiol. 7:388.
  21. Schmiel, D. H. and V. L. Miller. 1999. Bacterial phospholipases and pathogenesis. Microbes and Infection. 1:1103-1112.
  22. Revell, P. A. and V. L. Miller. 2001. Yersinia virulence: more than a plasmid. FEMS Microbiol. Letts. 205:159-164.
  23. Miller, V. L. 2002. Connections between transcriptional regulation and type III secretion? Curr. Opin. Microbiol. 5:211-215.
  24. Wolf-Watz, H. and V. L. Miller. 2003. Host-microbe interactions: bacteria. Bacteria and host: an eternally evolving interplay. Curr. Opin. Microbiol. 6:3-6.
  25. Ellison, D. W., M. B. Lawrenz, and V. L. Miller. 2004. Invasin and beyond: regulation of Yersinia virulence by RovA. Trends in Microbiol. 12:296-300.
  26. Ellison, D. W. and V. L. Miller. 2006. Regulation of virulence by members of the MarR/SlyA family. Curr. Opin. Microbiol. 9:153-159.

 

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