Department of Molecular Microbiology, Washington University in St. Louis, School of Medicine

 (photo)

William E. Goldman

Professor
Phone, office: (314)-362-2742
Phone, lab: (314)-362-2741
FAX: (314)-362-4879
email: goldman@borcim.wustl.edu

10240 McDonnell Pediatric Research Building
Box 8230 Department of Molecular Microbiology
Washington University School of Medicine
660 South Euclid Avenue
St. Louis, MO 63110-1093.

Research Interests | CV | Publications | Technology for licensing

Current Research Activities

Successful respiratory pathogens must be able to respond swiftly to a wide array of sophisticated defense mechanisms in the mammalian lung. In histoplasmosis, macrophages -- a first line of defense in the lower respiratory tract -- are effectively parasitized by Histoplasma capsulatum. This process depends on virulence factors produced as this "dimorphic" fungus undergoes a temperature-triggered conversion from a saprophytic mold form to a parasitic yeast form. One such molecule is a calcium-binding protein (CBP) that is secreted preferentially by the yeast form and is essential for Histoplasma virulence. The experiments to unravel CBP structure and function have relied heavily on our development of a telomeric shuttle plasmid that has been used for complementation cloning, gene disruptions, RNA interference, and reporter gene constructs. In addition, random insertional mutagenesis and transcriptional profiling with microarrays are helping us identify and characterize other genes involved in the pathogenesis of histoplasmosis.

Yersinia pestis also displays two temperature-regulated lifestyles, depending on whether it is colonizing a flea or mammalian host. Inhalation by humans leads to a rapid and overwhelming disease, and we are trying to understand the development of pneumonic plague by studying genes that are activated during the early stages of pulmonary colonization. We are also continuing studies of one of the virulence factors of Bordetella pertussis: tracheal cytotoxin (TCT) is a released fragment of peptidoglycan that is essential for triggering airway damage in whooping cough. TCT binds to a specific receptor that is a member of the peptidoglycan recognition protein (PGRP) family, and our current work is aimed at understanding host responses that include epithelial defense, cytopathology, and remodeling.

CV

Date of birth: July 7, 1955

Present position
1997 - present Professor
Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri

Education
1973 - 76 B.S. in Microbiology, Indiana University, Bloomington, Indiana
1976 - 80 Ph.D. in Bacteriology and Immunology, University of North Carolina Chapel Hill, North Carolina

Professional Experience and Appointments
1980 - 82 Postdoctoral Fellow,
Department of Microbiology and Immunology, Washington University School of Medicine, St. Louis, Missouri
1983 Instructor,
Department of Microbiology and Immunology, Washington University School of Medicine, St. Louis, Missouri
1983 - 90 Assistant Professor
Department of Microbiology and Immunology, Washington University School of Medicine, St. Louis, Missouri
1990 - 97 Associate Professor,
Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri

Honors and Awards
1976 Phi Beta Kappa; B.S. with Distinction and Departmental Honors
1981 - 83 NRSA Individual Postdoctoral Fellowship (NIH)
1996 - 2001 Burroughs-Wellcome Fund Scholar Award in Molecular Pathogenic Mycology
2002 Fellow of the American Academy of Microbiology
 Special Lectureships
1987 - 88 Foundation for Microbiology Lecturer
1993 Keynote speaker, Philadelphia Infection & Immunity Forum
1994 Keynote speaker, Midwest Microbial Pathogenesis Meeting
1997 Keynote speaker, Cold Spring Harbor Meeting on Microbial Pathogenesis and Host Response
1999 Plenary lecture, Oral Immunology/Microbiology Research Group Annual Meeting
1999 American Society for Microbiology Division D Lecturer
2000 Featured speaker, Premières Rencontres Saint Louis-Pasteur, Institut Pasteur
2001 Karl Beyer Visting Professorship, University of Wisconsin
2003 University of Georgia (Department of Microbiology 50th anniversary symposium)
2006 Featured speaker, ASM Conference on Dimorphic Fungal Pathogens


Professional Responsibilites
Editorial
1987 - 2001 Editorial Board, Infection and Immunity
1997 - present Editorial Board, Current Opinion in Microbiology
1998 Section Editor, Current Opinion in Microbiology (Host-Microbe Interactions)
1999 - present Editorial Board, Cellular Microbiology
2000 - present Editorial Board, Trends in Microbiology
2001 - present Editor, Molecular Microbiology
2001 - present "Faculty of 1000" Section Co-Head (Cellular Microbiology and Pathogenesis)
  Ad hoc reviewer: Cell, Science, Proceedings of the National Academy of Sciences USA, Genes & Development, Journal of Experimental Medicine, Journal of Clinical Investigation, Molecular Microbiology, Journal of Bacteriology, Journal of Immunology, Genetics, Journal of Leukocyte Biology, Gene, Biochemistry, Journal of Infectious Diseases, Clinical Microbiology Reviews, Medical Mycology, American Journal of Respiratory Cell and Molecular Biology, Eukaryotic Cell
 Grant/Program Review
1990 - 94 Reviewer, NIH Biological Sciences Study Section, Subcommittee 3
1991, 2002 Ad hoc reviewer, NIH Bacteriology and Mycology Study Sections
1998 Ad hoc member, NIAID Board of Scientific Counselors
2005 - 2008 Advisory Committee, Burroughs Wellcome Fund
  Ad hoc external grant reviewer, NIH and Veterans Affairs Medical Research Service
 Conferences
1998 - 2000 Organizer, Pertussis Group Meeting (at the annual ASM Conference)
1998 Co-organizer of the 5th annual Midwest Microbial Pathogenesis Meeting
2000 Chair of FASEB Research Conference on Microbial Pathogenesis: Mechanisms of Infectious Diseases
2001 ASM General Meeting Colloquium Advisory Committee
2002 Chair of Gordon Research Conference on Microbial Toxins and Pathogenicity
2002 Planning Committee, International Symposium on Pertussis
2003 American Academy of Microbiology Colloquium on Microbiology in the 21st Century
2003 American Academy of Microbiology Colloquium on Pathogen Genomics
2004 American Academy of Microbiology Colloquium on Environmental Microorganisms as Human Pathogens
2004- present ASM Conferences Committee
2005 ASM-NIH Workshop on Basic Bacterial Research
2006 Scientific Committee (Chair), ASM Conference on Dimorphic Fungal Pathogens

Invited Lectures (International Conferences)
1984, 88, 90, 2002 International Symposium on Pertussis
1985, 87, 90, 92, 94-97, 99, 2001 American Society for Microbiology General Meeting 
1988 International Workshop on Bordetella pertussis
1992 Gordon Conference on Fungal Metabolism
1992, 98 Gordon Conference on Microbial Toxins and Pathogenesis
1993 Bristol Myers-Squibb Symposium on Infectious Disease Research
1993 NIAID Molecular Medical Mycology Workshop (discussion leader)
1994 Keystone Symposium on Molecular Events in Microbial Pathogenesis
1994 International Mycological Congress
1994 Karolinska Institute Symposium on Molecular Pathogenesis in Infections and Tumors
1995 International Symposium on Topics in Mycology: Host-Fungus Interplay
1995 International Workshop on Molecular Genetic Approaches to the Study of Fungi
1995 European Workshop Conference on Bacterial Protein Toxins
1996 Transatlantic Airway Conference on Bacterial Interactions with Airway Cells and Secretions
1996 Table Ronde Roussel on Contribution of Molecular Pathogenesis to the Discovery of New Antiinfectious Drugs
1996 FASEB Research Conference on Principles in Viral, Bacterial, Fungal, and Protozoal Pathogenesis
1997, 2003 Congress of the International Society for Human and Animal Mycology
1997 International Congress of Biochemistry and Molecular Biology
1999 Cold Spring Harbor Meeting on Microbial Pathogenesis and Host Response
1998 Keystone Symposium on Opportunistic Infections in AIDS
1998 American Lung Association - American Thoracic Society International Conference
1998, 2002 Woods Hole Molecular Mycology Course
1999 Society for General Microbiology (U.K.) Symposium on Respiratory Pathogens
1999 Interscience Conference on Antimicrobial Agents and Chemotherapy
1999 National Academy of Sciences Colloquium on Virulence and Defense in Host-Pathogen Interactions
2001 Gordon Conference on Molecular Mechanisms of Microbial Adhesion and Signal Transduction
2002 Gordon Conference on Biology of Host-Parasite Interactions
2002 FASEB Research Conference on Microbial Pathogenesis: Mechanisms of Infectious Diseases
2003 Woods Hole course on Biology of Parasitism 
2003 Awaji International Forum on Infection and Immunity
2003 Frontier Studies and International Networking of Genetic Resources in Pathogenic Microorganisms (Japan)
2004 Microbes of High Pathogenic Potential - Strategies for Detection, Controlling and Eradication (Germany)
2004 St. Jude/PIDS Pediatric Microbial Research Conference
2004 Vibrio fischeri - Euprymna scolopes Symbiosis Symposium
2005 International Meeting on Paracoccidioidomycosis (Brazil)
2006 ASM Biodefense Meeting (Washington, D.C.)
2006 Burroughs Wellcome Fund Conference on Infectious Diseases (discussion leader)
2006 International Symposium on Yersinia

Bibliography

  1. Goldman, W.E., and J.B. Baseman. 1980. Selective isolation and culture of a proliferating epithelial cell population from the hamster trachea. In Vitro 16:313-319.
  2. Goldman, W.E., and J. B. Baseman. 1980. Glycoprotein secretion by cultured hamster trachea epithelial cells: A model system for in vitro studies of mucus synthesis. In Vitro 16:320-329.
  3. Baseman, J.B., N.S. Hayes, W.E. Goldman, and A.M. Collier. 1980. Mucus and surfactant synthesis and secretion by cultured hamster respiratory cells. Environmental Health Perspectives 35:139-146.
  4. Goldman, W.E., D.G. Klapper, and J.B. Baseman. 1982. Detection, isolation, and analysis of a released Bordetella pertussis product toxic to cultured tracheal cells. Infection and Immunity 35: 782-794.
  5. Goldman, W.E., G. Goldberg, L.H. Bowman, D. Steinmetz, and D. Schlessinger. 1983. Mouse rDNA: Sequences and evolutionary analysis of spacer and mature RNA regions. Molecular and Cellular Biology 3:1488-1500.
  6. Bowman, L.H., W.E. Goldman, G.I. Goldberg, M.B. Hebert, and D. Schlessinger. 1983. Location of the initial cleavage sites in mouse pre-rRNA. Molecular and Cellular Biology 3:1501-1510.
  7. Goldman, W.E., and L.A. Herwaldt. 1985. Bordetella pertussis tracheal cytotoxin. Developments in Biological Standardization 61:103-111.
  8. Vincent, R.D., R. Goewert, W.E. Goldman, G.S. Kobayashi, A.M. Lambowitz, and G. Medoff. 1986. Classification of Histoplasma capsulatum isolates by restriction fragment polymorphisms. Journal of Bacteriology 165:813-818.
  9. Goldman, W.E. 1986. Bordetella pertussis tracheal cytotoxin: Damage to the respiratory epithelium. pp. 65-69, in L. Leive (ed.), Microbiology -- 1986. American Society for Microbiology, Washington, D.C.
  10. Eissenberg, L.G., and W. E. Goldman. 1987. Histoplasma capsulatum fails to trigger release of superoxide from macrophages. Infection and Immunity 55:29-34.
  11. Klimpel, K.R., and W. E. Goldman. 1987. Isolation and characterization of spontaneous avirulent variants of Histoplasma capsulatum. Infection and Immunity 55:528-533.
  12. Rosenthal, R.S., W. Nogami, B.T. Cookson, W.E. Goldman, and W.J. Folkening. 1987. Major fragment of soluble peptidoglycan released from growing Bordetella pertussis is tracheal cytotoxin. Infection and Immunity 55:2117-2120.
  13. Eissenberg, L.G., P.H. Schlesinger and W.E. Goldman. 1988. Phagosome-lysosome fusion in P388D1 macrophages infected with Histoplasma capsulatum. Journal of Leukocyte Biology 43:483-491.
  14. Worsham, P.L., and W.E. Goldman. 1988. Quantitative plating of Histoplasma capsulatum without addition of conditioned medium or siderophores. Journal of Medical and Veterinary Mycology 26:137-143.
  15. Worsham, P.L., and W.E. Goldman. 1988. Selection and characterization of ura5 mutants of Histoplasma capsulatum. Molecular and General Genetics 214:348-352.
  16. Gentry-Weeks, C.R., B.T. Cookson, W.E. Goldman, R.B. Rimler, S. Porter, and R. Curtiss III. 1988. Dermonecrotic toxin and tracheal cytotoxin: Putative virulence factors of Bordetella avium. Infection and Immunity 56:1698-1707.
  17. Klimpel, K.R., and W.E. Goldman. 1988. Cell walls from avirulent variants of Histoplasma capsulatum lack a-(1,3)-glucan. Infection and Immunity 56:2997-3000.
  18. Eissenberg, L.G., and W. E. Goldman. 1988. Fusion of lysosomes with phagosomes containing Histoplasma capsulatum: Use of fluoresceinated dextran. Advances in Experimental Medicine and Biology 239:53-61.
  19. Goldman, W.E. 1988. Tracheal cytotoxin of Bordetella pertussis. pp. 231-246, in A.C. Wardlaw and R. Parton (eds.), Pathogenesis and Immunity in Pertussis. John Wiley & Sons, Sussex, England.
  20. Goldman, W.E., and B.T. Cookson. 1988. Structure and functions of the Bordetella tracheal cytotoxin. Tokai Journal of Experimental and Clinical Medicine 13 (Suppl.):187-191.
  21. Cookson, B.T., A.N. Tyler, and W.E. Goldman. 1989. Primary structure of the peptidoglycan-derived tracheal cytotoxin of Bordetella pertussis. Biochemistry 28:1744-1749.
  22. Cookson, B.T., H.-L. Cho, L.A. Herwaldt, and W.E. Goldman. 1989. Biological activities and chemical composition of purified tracheal cytotoxin of Bordetella pertussis. Infection and Immunity 57:2223-2229.
  23. Cookson, B.T., D.E. Berg, and W.E. Goldman. 1990. Mutagenesis of Bordetella pertussis with transposon Tn5tac1: Conditional expression of virulence-associated genes. Journal of Bacteriology 172:1681-1687.
  24. Worsham, P.L., and W.E. Goldman. 1990. Development of a genetic transformation system for Histoplasma capsulatum: Complementation of uracil auxotrophy. Molecular and General Genetics 221:358-362.
  25. Goldman, W.E., J.L. Collier, B.T. Cookson, G.R. Marshall, and K.M. Erwin. 1990. Tracheal cytotoxin of Bordetella pertussis: Biosynthesis, structure and specificity. pp. 5-12, in C.R. Manclark (ed.), Proceedings of the Sixth International Symposium on Pertussis. Department of Health and Human Services, U.S. Public Health Service, Bethesda, MD.
  26. Cookson, B.T., T. Tomcsanyi, D.E. Berg, and W.E. Goldman. 1990. A strategy for studying pathogenic mechanisms: Transposon insertion mutations with conditional phenotypes. pp. 243-250, in C.R. Manclark (ed.), Proceedings of the Sixth International Symposium on Pertussis. Department of Health and Human Services, U.S. Public Health Service, Bethesda, MD.
  27. Wilson, R., R. Read, M. Thomas, A. Rutman, K. Harrison, V. Lund, B. Cookson, W. Goldman, H. Lambert, and P. Cole. 1991. Effects of Bordetella pertussis infection on human respiratory epithelium in vivo and in vitro. Infection and Immunity 59:337-345.
  28. Eissenberg, L.G., J.L. West, J.P. Woods, and W.E. Goldman. 1991. Infection of P388D1 macrophages and respiratory epithelial cells by Histoplasma capsulatum: Selection of avirulent variants and their potential role in persistent histoplasmosis. Infection and Immunity 59:1639-1646.
  29. Erwin, K.M., J.L. Collier, G.R. Marshall, and W.E. Goldman. 1991. Molecular structural requirements for tracheal cytotoxin function. pp. 803-805, in E. Giralt and D. Andreu (eds.), Peptides 1990. Proceedings of the Twenty-First European Peptide Symposium. ESCOM Science Publishers B.V.
  30. Eissenberg, L.G., and W.E. Goldman. 1991. Histoplasma variation and adaptive strategies for parasitism: New perspectives on histoplasmosis. Clinical Microbiology Reviews 4:411-421.
  31. Kersulyte, D., J.P. Woods, E.J. Keath, W.E. Goldman, and D.E. Berg. 1992. Diversity among clinical isolates of Histoplasma capsulatum detected by PCR with arbitrary primers. Journal of Bacteriology 174:7075-7079.
  32. Woods, J.P., and W.E. Goldman. 1992. In vivo generation of linear plasmids with addition of telomeric sequences by Histoplasma capsulatum. Molecular Microbiology 6:3603-3610.
  33. Erwin, K.M., J.L. Collier, G.R. Marshall, and W.E. Goldman. 1992. Synthesis and biological activity of peptide analogs of Bordetella pertussis tracheal cytotoxin. pp. 931-932, in J.A. Smith and J.E. Rivier (eds.), Peptides. Chemistry and Biology. Proceedings of the Twelfth American Peptide Symposium. ESCOM Science Publishers B.V.
  34. Woods, J.P., D. Kersulyte, W.E. Goldman, and D.E. Berg. 1993. Fast DNA isolation from Histoplasma capsulatum: Methodology for arbitrary primer polymerase chain reaction-based epidemiological and clinical studies. Journal of Clinical Microbiology 31:463-464.
  35. Woods, J.P., and W.E. Goldman. 1993. Autonomous replication of foreign DNA in Histoplasma capsulatum: The role of native telomeric sequences. Journal of Bacteriology 175:636-641.
  36. Luker, K.E., J.L. Collier, E.W. Kolodziej, G.R. Marshall, and W.E. Goldman. 1993. Bordetella pertussis tracheal cytotoxin and other muramyl pep tides: Distinct structure-activity relationships for respiratory epithelial cytopathology. Proceedings of the National Academy of Sciences U.S.A. 90:2365-2369.
  37. Eissenberg, L.G., W.E. Goldman, and P.H. Schlesinger. 1993. Histoplasma capsulatum modulates the acidification of phagolysosomes. Journal of Experimental Medicine 177:1605-1611.
  38. Heiss, L.N., S.A. Moser, E.R. Unanue, and W.E. Goldman. 1993. Interleukin-1 is linked to the respiratory epithelial cytopathology of pertussis. Infection and Immunity 61:3123-3128.
  39. Heiss, L.N., J.R. Lancaster, Jr., J.A. Corbett, and W.E. Goldman. 1994. Epithelial autotoxicity of nitric oxide: Role in the respiratory cytopathology of pertussis. Proceedings of the National Academy of Sciences U.S.A. 91:267-270.
  40. Cundell, D.R., K. Kanthakumar, G.W. Taylor, W.E. Goldman, T. Flak, P.J. Cole, and R. Wilson. 1994. The effect of tracheal cytotoxin from Bordetella pertussis on human neutrophil function in vitro. Infection and Immunity 62:639-643.
  41. Goldman, W.E. 1994. The broadening spectrum of bacterial toxin - target cell interactions. Infectious Agents and Disease 2:169-172.
  42. Heiss, L.N., T.A. Flak, J.R. Lancaster, Jr., M.L. McDaniel, and W.E. Goldman. 1994. Nitric oxide mediates Bordetella pertussis tracheal cytotoxin damage to the respiratory epithelium. Infectious Agents and Disease 2:173-177.
  43. Eissenberg, L.G., and W.E. Goldman. 1994. The interplay between Histoplasma capsulatum and its host cells. Baillire's Clinical Infectious Diseases 1:265-283.
  44. Luker, K.E., A.N. Tyler, G.R. Marshall, and W.E. Goldman. 1995. Tracheal cytotoxin structural requirements for respiratory epithelial damage in pertussis. Molecular Microbiology 16:733-743.
  45. Heiss, L.N., K.E. Luker, and W.E. Goldman. 1995. Muramyl peptides as exotoxins: Bordetella tracheal cytotoxin. pp. 407-423, in J. Moss, B. Iglewski, M. Vaughan, and A.T. Tu (eds.), Handbook of Natural Toxins, Volume 8: Bacterial Toxins and Virulence Factors in Disease. Marcel Dekker, Inc., New York.
  46. Goldman, W.E. 1995. Molecular genetic technology transfer to pathogenic fungi. Archives of Medical Research 26:437-440.
  47. Palmer, K.L., W.E. Goldman, and R.S. Munson, Jr. 1996. An isogenic hemolysin-deficient mutant of Haemophilus ducreyi lacks the ability to produce cytopathic effect on human foreskin fibroblasts. Molecular Microbiology 21:13-19.
  48. Eissenberg, L.G., S. Poirier, and W.E. Goldman. 1996. Phenotypic variation and persistence of Histoplasma capsulatum yeasts in host cells. Infection and Immunity 64:5310-5314.
  49. Flak, T.A., and W.E. Goldman. 1996. Autotoxicity of nitric oxide in airway disease. American Journal of Respiratory and Critical Care Medicine 154:S202-S206.
  50. Eissenberg, L.G., S.A. Moser, and W.E. Goldman. 1997. Alterations to the cell wall of Histoplasma capsulatum yeasts during infection of macrophages and epithelial cells. Journal of Infectious Diseases 175:1538-1544.
  51. Batanghari, J.W., and W.E. Goldman. 1997. Calcium dependence and binding in cultures of Histoplasma capsulatum. Infection and Immunity 65:5257-5261.
  52. Batanghari, J.W., and W.E. Goldman. 1997. A potential role for calcium in Histoplasma parasitism. pp. 65-72, in H. Vanden Bossche, D.A. Stevens, and F.C. Odds (eds.), Host-Fungus Interplay. National Foundation for Infectious Diseases, Bethesda, Maryland.
  53. Batanghari, J.W., G.S. Deepe, E. Di Cera, and W.E. Goldman. 1998. Histoplasma acquisition of calcium and expression of CBP1 during intracellular parasitism. Molecular Microbiology 27:531-539.
  54. Patel, J.B., J.W. Batanghari, and W.E. Goldman. 1998. Probing the yeast phase-specific expression of the CBP1 gene in Histoplasma capsulatum. Journal of Bacteriology 180:1786-1792.
  55. Woods, J.P., E.L. Heinecke, and W.E. Goldman. 1998. Electrotransformation and expression of bacterial genes encoding hygromycin phosphotransferase and b-galactosidase in the pathogenic fungus Histoplasma capsulatum. Infection and Immunity 66:1697-1707.
  56. Flak, T.A., and W.E. Goldman. 1998. Muramyl peptide probes derived from tracheal cytotoxin of Bordetella pertussis. Analytical Biochemistry 264:41-46.
  57. Woods, J.P, D.M. Retallack, E.L. Heinecke, and W.E. Goldman. 1998. Rare homologous gene targeting in Histoplasma capsulatum: Disruption of the URA5Hc gene by allelic replacement. Journal of Bacteriology 180:5135-5143.
  58. Goldman, W.E., L. Babiuk, and D.G. Russell. 1998. Host-microbe interactions: fungi/viruses/parasites. Current Opinion in Microbiology 1:377-380.
  59. Kwon-Chung, K.J., W.E. Goldman, B. Klein, and P.J. Szaniszlo. 1998. Fate of transforming DNA in pathogenic fungi. Medical Mycology 36 (Suppl. 1):38-44
  60. Flak, T.A., W.E. Goldman. 1999. Signaling and cellular specificity of airway nitric oxide production in pertussis. Cellular Microbiology 1:51-60
  61. Goldman, W.E. 1999. Looking for a few good mutants. Science 285:539-541.
  62. Flak, T.A., L.N. Heiss, W.E. Goldman. 2000. Synergistic epithelial responses to endotoxin and a naturally occurring muramyl peptide. Infection and Immunity 68:1235-1242.
  63. Kügler, S., T.S. Sebghati, L.G. Eissenberg, and W.E. Goldman. 2000. Phenotypic variation and intracellular parasitism by Histoplasma capsulatum. Proceedings of the National Academy of Sciences U.S.A. 97:8794-8798.
  64. Sebghati, T.S., J.T. Engle, and W.E. Goldman. 2000. Intracellular parasitism by Histoplasma capsulatum: Fungal virulence and calcium dependence. Science 290:1368-1372.
  65. Beckett, C.S., J.A. Loughman, K.A. Karberg, G. Donato, W.E. Goldman, and R.G. Kranz. 2000. Four genes are required for the system II cytochrome c biogenesis pathway in Bordetella pertussis, a unique bacterial model. Molecular Microbiology 38:465-481.
  66. Kügler, S., B. Young, V.L. Miller, and W. E. Goldman. 2000. Monitoring phase-specific gene expression in Histoplasma capsulatum with telomeric GFP fusion plasmids. Cellular Microbiology 2:549-560.
  67. Magrini, V., and W.E. Goldman. 2001. Molecular mycology: A genetic toolbox for Histoplasma capsulatum. Trends in Microbiology 9:541-546.
  68. Liu, H., T.R. Cottrell, L.M. Pierini, W.E. Goldman, and T.L. Doering. 2002. RNA interference in the pathogenic fungus Cryptococcus neoformans. Genetics 60: 463-470.
  69. Leevy, W.M., G. Donato, W.E. Goldman, P.H. Schlesinger, and G.W. Gokel. 2002 Synthetic hydraphile channels of the appropriate length kill E. coli. Journal of the American Chemical Society 124:9022-9023
  70. Kaneko, T., W.E. Goldman, P. Mellroth, H. Steiner, K. Fukase, S. Kusumoto, W. Harley, A Fox, D. Golenbock, and N. Silverman. 2004. Monomeric and polymeric gram-negative peptidoglycan but not purified LPS stimulate the Drosophila IMD pathway. Immunity 20:637-649
  71. Magrini, V., W.C. Warren, J. Wallis, W.E. Goldman, J. Xu, E.R. Mardis, and J. D. McPherson. 2004. Fosmid-based physical mapping of the Histoplasma capsulatum genome. Genome Research 14:1603-1609.
  72. Rappleye, C.A., J.T. Engle, and W.E. Goldman. 2004. RNA interference in Histoplasma capsulatum demonstrates a role for a-(1,3)-glucan in virulence. Molecular Microbiology 53:153-165.
  73. Koropatnick, T.A., J.T. Engle, M.A. Apicella, E.V. Stabb, W.E. Goldman, and M.J. McFall-Ngai. 2004. Microbe factor-mediated development in a host-bacterial mutualism. Science 306:1186-1188.
  74. Mellroth, P., J. Karlsson, J. Håkansson, N. Schultz, W.E. Goldman, and H. Steiner. 2005. Ligand induced dimerization of Drosophila peptidoglycan recognition proteins. Proceedings of the National Academy of Sciences U.S.A. 102:6455-6460.
  75. Goldman, W.E., and C.A. Rappleye. 2005. RNA interference as a tool for studying fungal pathogens. Nova Acta Leopoldina 92:141-145.
  76. Lathem, W.W., S.D. Crosby, V.L. Miller, and W.E. Goldman. 2005. Progression of primary pneumonic plague: A mouse model of infection, pathology, and bacterial transcriptional activity. Proceedings of the National Academy of Sciences U.S.A. 102:17786-17791.
  77. Swaminathan, C.P., P.H. Brown, A. Roychowdhury, Q. Wang, R. Guan, N. Silverman, W.E. Goldman, G.-J. Boons, and R.A. Mariuzza. 2006. Dual strategies for peptidoglycan discrimination by peptidoglycan recognition proteins (PGRPs). Proceedings of the National Academy of Sciences U.S.A. 103:684-689.
  78. Lim J.H., M.S. Kim, H.E. Kim, T. Yano, Y. Oshima, K. Aggarwal, W.E. Goldman, N. Silverman, S. Kurata, and B.H. Oh. 2006. Structural basis for preferential recognition of diaminopimelic acid-type peptidoglycan by a subset of peptidoglycan recognition proteins. Journal of Biological Chemistry 281:8286-8295.
  79. Rappleye, C.A., and W.E. Goldman. 2006. Defining virulence genes in the dimorphic fungi. Annual Review of Microbiology 60:281-303.
  80. Mielcarek, N., A.-S. Debrie, D. Raze, J. Bertout, A. Ben Younes, J. Engle, W.E. Goldman, and C. Locht. 2006. Live attenuated Bordetella pertussis as a highly efficient single-dose mucosal vaccine against whooping cough. PLoS Pathogens 2:e65.
  81. Kaneko, T., T. Yano, K. Aggarwal, J.H. Lim, K. Ueda, Y. Oshima, C. Peach, D. Erturk-Hasdemir, W.E. Goldman, B.H. Oh, S. Kurata and N. Silverman. 2006. PGRP-LC and PGRP-LE play essential yet distinct roles in the Drosophila immune response to monomeric DAP-type peptidoglycan. Nature Immunology 7:715-723.
  82. Cathelyn, J., S.D. Crosby, W.W. Lathem, W.E. Goldman, and V.L. Miller. RovA, a global regulator of Yersinia pestis, specifically required for bubonic plague. 2006. Proceedings of the National Academy of Sciences U.S.A. 103:13514-13519.
  83. Marion, C.L., C.A. Rappleye, J.T. Engle, and W.E. Goldman. 2006. An a-(1,4)-amylase is essential for a-(1,3)-glucan production and virulence in Histoplasma capsulatum. Molecular Microbiology 62:970-983.
  84. Cloud-Hansen, K.A., S.B. Peterson, E.V. Stabb, W.E. Goldman, M.J. McFall-Ngai, and J. Handelsman. 2006. Breaching the great wall: Peptidoglycan and microbial interactions. Nature Reviews Microbiology 4:710-716.
  85. Lathem, W.W., V.L. Miller, and W.E. Goldman. 2007. A plasminogen-activating protease specifically controls the development of primary pneumonic plague. Science 26:509-513.
  86. Rappleye, C.A., L.G. Eissenberg, and W.E. Goldman. 2007. Histoplasma capsulatum a-(1,3)-glucan blocks recognition by the macrophage b-glucan receptor. Proceedings of the National Academy of Sciences U.S.A. 104:1366-1370.

Technology for Licensing

Patents

Goldman, W.E. 1994. Method for the treatment of pertussis. US patent number No. 5,317,040.

Lathem, W.W., V.L. Miller, and W.E. Goldman. Methods of treating pneumonic plague. Provisional patent application (pending).

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